中文摘要：

目的：探讨脂质灌注对大鼠血浆抵抗素和ghrelin的影响。方法：采用正糖钳夹技术，在钳夹前后分别测定生理盐水对照组和脂质灌注组血浆抵抗素和ghrelin浓度，并用[^3H]-葡萄糖作为示踪剂测定外周组织和肝糖的代谢。结果：脂质灌注组大鼠血浆游离脂肪酸（FFA）明显增加（P〈0．01），葡萄糖输注率（GIR）明显降低（P〈0．01）。对照组肝糖产率（HGP）明显被抑制（88％）。脂质输注组胰岛素对HGP的抑制作用明显减弱。在钳夹期间。脂质组与对照组比葡萄糖清除率（GRd）轻度降低。在正糖钳夹术结束时，对照组血浆ghrelin水平与钳夹前相比明显降低（P〈0．05）。4h的脂质灌注也引起了血浆ghrelin浓度的明显下降（P〈0．05），但是在钳夹结束时和对照组比没有明显差异。相关性分析表明空腹血浆ghrelin水平与空腹胰岛素和血糖呈明显负相关（r=-0．52和r=-0．61。P〈0．05）。脂质灌注后大鼠血浆抵抗素水平较灌注前和对照组明显升高（P〈0．01），空腹血浆抵抗素浓度与空腹FFA（r=0．68，P〈0．01）、血糖（r=0．66，P〈0．01）呈明显正相关。结论：脂质灌注诱导了肝脏和外周的胰岛素抵抗，抵抗素在胰岛素抵抗的形成中可能具有重要作用。高胰岛素血症，而不是游离脂肪酸，降低了大鼠循环ghrelin水平。

英文摘要：

AIM： To investigate the effects of lipid - induced insulin resistance on plasma resistin and ghrelin levels in awake rats. METHODS： A hyperinsalinaemic- euglycaemic clamp was established in awake chronically catheterized rats. Two groups of rats were studied either with a 4 h intraarterial infusion of lipid/hepafin or saline. Insulin - mediated peripheral and hepatic glucose metabolism was assessed by insulin clamp combined with [^3H] - glucose infusion, and plasma resistin and ghrelin concentrations were examined before and -after clamp with ELISA. RESULTS： During clamp, there was a significant increase in plasma free fatty acid （FFA, P 〈 0.01） and significant decrease in glucose infusion rates （GIR, P 〈 0.01） in lipid - infused group. At the end of clamp study, the hepatic glucose production （HGP） in controls rats was significantly suppressed to - 88%. The suppressive effect of insulin on HGP was significantly blunted in the lipid - infused rats. Plasma resistin levels in the lipid infusion rats were markedly elevated, compared to control rats and before infusion. A lipid infusion of 4 h also caused a significant decrease in plasma ghrelin concentration, when compared to -basal levels. But at the end of clamp, plasma ghrelin levels in control and lipid- infused rats had no difference. Fasting plasma resistin concentrations were correlated with plasma FFA levels （ r = 0.68, P 〈 0.01 ）, blood glucose （ r = 0.66, P 〈 0.01 ）, and fasting plasma ghrelin concentration was negatively correlated with fasting plasma insulin levels （ r = - 0.52, P 〈 0.05） and blood glucose （ r = - 0.61, P 〈 0.05）. CONCLUSION： Lipid - infusion induces a hepatic and peripheral insulin resistance, and circulating resistin is likely to play a major role in insulin resistance. Hyperinsulinemia, but not FFA, decreases circtdating ghrelin in rats.