中文摘要：

本试验扩增并克隆测定了牦牛2个α-珠蛋白基因间区域的序列．根据所测结果及人、鼠、山羊、马和黄牛的α-珠蛋白基因序列比对中的保守区设计了2对特异性引物，扩增并克隆测定了牦牛α-蛋白的上、下游基因序列．将测定的牦牛α-珠蛋白基因间区域序列进行同源性搜索，发现没有任何二个基因与它同源．上、下游基因的测序结果表明，上游基因为牦牛α1-珠蛋白基因，下游基因为α2-珠蛋白基因．这表明牦牛的2个α-珠蛋白基因在染色体上紧密排列，其中α1-珠蛋白基因在前，α2-珠蛋白基因在后．

英文摘要：

Two differential primers were designed from the sequence of yak＇s two α-globin genes＇s intergenic region which was cloned and sequenced, and from the conserved region of human, mouse, goat, horse and cattle＇s α-globin gene for confirming the order of yak＇s two α-globin genes. There was no other gene in the intergenic region of yak＇s two α-globin genes after blasting. The upstream gene was identified as yak＇s α1-globin gene, while the downstream gene was identified as yak＇s α2-globin gene after cloning and sequencing. These indicate that the yak＇s two α-globin genes are located in the chromosome tightly, α1-globin gene in the front and α2-globin gene at the back,