中文摘要：

采用RT-PCR技术从本生烟中分别克隆获得水杨酸结合蛋白2（salicylicacid-bind-ingprotein2，SABP2）和水杨酸羧基转甲基酶（SAmethyltransferase，SAM丁）基因的全长序列．利用Gateway定向克隆系统，分别将SABP2和SAMT部分序列片段插入到烟草脆裂病毒（Tobaccorattlevirus，TRV）载体中，构建了重组病毒载体pTRV2-NbSABP2和pTRV2-NbSAMT．将重组载体转化农杆菌GV2260，通过TRV病毒诱导的基因沉默（virus-inducedgenesilencing，VIGS）系统，分别抑制本生烟SABP2和SAMT基因的表达．与TRV2空载体相比，pTRV2-NbSABP2载体浸润的植株生长矮小，叶柄基部和叶脉褐化，叶片向下塌陷；pTRV2-NbSAMT载体浸润的植株生长正常，无明显的表型改变．

英文摘要：

The complete nucleotide sequence of salicylic acidbinding protein 2（SABP2） and SA methyl transferase （SAMT） were obtained from Nicotiana benthamiana using RTPCR （reverse transcription polymerase chain reaction） method in this study. Then partial fragments of SABP2 and SAMT were inserted into tobacco rattle virus （TRV） vector through directional TOPOCloning of Gateway System respectively. Thus the recombinant pTRV2- NbSABP2 and pTRV2-NbSAMT vectors were constructed. They were transformed into cells of Agrobacterium tumefacien GV2260, and the expression of Nb SABP2 and NbSAMT were suppressed through TRV virusinduced gene silencing （VIGS） system. When the plants were infiltrated with pTRV2-NbSABP2, they grew smaller and the leaves collapsed, with browning at the base of petiole or vein compared with TRV2 empty vector Whereas plants infiltrated with pTRV2-NbSAMT grew normal and there was no obvious difference of phenotype.