中文摘要：

从感染芜菁皱缩病毒（TCV）的拟南芥中提取总RNA,通过反转录PCR（RT-PCR）扩增得到TCV的外壳蛋白基因P38.在目的基因ORF框两边引入酶切位点BamHⅠ和SacⅠ,并连接到表达载体pET30-a上,导入大肠杆菌BL21（DE3）中,37℃经0.5mM/L IPTG诱导6h后获得了分子量约为48kD的融合蛋白.经Ni-NTA纯化目的蛋白,注射大白兔制备抗血清.Western blot结果表明制备好的抗血清能成功检测感染TCV的拟南芥.

英文摘要：

The nucleotide sequence of the coat protein（P38）gene of Turnip crinkle virus was obtained using RT-PCR fromArabidopsis thalianainfected with TCV.Restriction enzyme cutting sites of BamHⅠ and SacⅠ were introduced to the ORF fragment of P38.Then the fragment was linked together with PET30-a and transformed into E.coli BL21（DE3）.The target fusion protein（48kD）was induced by0.5mM/L IPTG at 37℃for 6h.The expressed protein was purified by Ni-NTA and anti-P38 polyclonal antibody was prepared using rabbit.Western blot results showed that the antiserum could be used to detect the expression of P38 in infected Arabidopsis thaliana.