中文摘要：

目的探讨乙酰辅酶A羧化酶B（ACACB）基因rs2268388多态性与我国昆明地区汉族人群糖尿病慢性肾脏疾病（CKD）的相关性。方法选取T2DM患者248例,根据两次UAlb/Cr或24hUAlb结果分为单纯T2DM组85例、CKD1组99例及CKD2组64例,另选取健康对照（NC）组70名。采用Taqman PCR（Three-star）法对各组ACACB基因rs2268388多态性进行检测,并比较分析各组基因型、等位基因频率、风险因素及相关临床和生化指标。结果 （1）单纯T2DM、CKD各组C/T基因型频率均低于NC组（Z=-2.391,P=0.017）;CKD1组和CKD2组ACACB基因rs2268388T/T基因型频率高于单纯T2DM组（Z=-2.522,P=0.012;Z=-4.981,P=0.001）;CKD1组和CKD2组T/T基因型频率及各组等位基因频率比较,差异无统计学意义（P〉0.05）。（2）Logistic回归分析表明,糖尿病病程、rs2268388T/T基因型、高TG、高HbA1c及高SUA是CKD发生的危险因素。结论在昆明地区汉族人群中,ACACB基因rs2268388T/T基因型可能与CKD发生相关。

英文摘要：

Objective To explore the association of the rs2268388 polymorphism of ACACB gene with chronic kidney disease（CKD）in diabetes in Kunming Han Chinese population.Methods A total of248T2 DM patients and 70 healthy controls were enrolled in this study.All the T2 DM patients were divided into three groups：simple T2 DM group（UAlb/Cr〈30mg/24 h,n=85）,CKD1group（UAlb/Cr 30~299mg/24 h,n=99）and CKD2group（UAlb/Cr〉300mg/24 h,n=64）.The genotype was detected by Taqman PCR（Three-star）.The genotype and allele frequencies and related clinic characteristics were compared among groups.Results（1）C/T genotype frequencies in simple T2DM、CKD1 and CKD2 group were significantly lower than in NC group（Z=-2.391,P=0.017）.T/T genetype frequencies of ACACB gene in CKD1 and simple T2 DM group were significantly higher than in simple T2 DM group（Z=-2.522,P =0.012;Z=-4.981,P =0.001）.The difference of T/T genetype frequency was not significant between CKD2 and CKD1 group（P〉0.05）.（2）Logistic regression analysis showed that in patients with T2 DM,diabetic duration,rs2268388 T/T genotype,higher TG,higher HbA1 c and higher SUA were risk factors for CKD.Conclusion rs2268388T/T genotype of ACACB gene may be related with CKD development in Kunming Han Chinese population.