中文摘要：

鳗鲡疱疹病毒（Anguillae herpesvirus,AngHV）是淡水鳗鲡的主要病毒性疾病病原之一。根据鳗鲡疱疹病毒（AngHV-1）的基因组序列（GenBank：NC-013668）设计引物,扩增鳗鲡疱疹病毒福建株（AngHV-FJ）ORF95基因的开放阅读框序列,克隆至pMD19-T载体;经限制性内切酶酶切和测序鉴定,进一步将其克隆至原核表达载体pET-32a,构建了表达质粒32a-ORF95,将其转化表达菌株BL21（DE3）,经IPTG诱导,实现了ORF95在大肠杆菌中的高效表达。在0.1mmol.L-1 IPTG、23℃诱导14h的条件下,可溶性ORF95蛋白的表达量较高,经镍柱纯化获得了高纯度的融合蛋白。

英文摘要：

Anguillae herpesvirus（AngHV）is one of the major viral pathogen of freshwater eels.In this study,the open reading frame of AngHV-FJ ORF95 gene was synthesized according to the gene sequence of AngHV-1 provided by GenBank：（NC-013668）,and then cloned into the pMD19-T vector.After restriction digestion and sequence verification,the ORF95 gene was inserted into the prokaryotic expression vector pET-32a to construct the recombinant expression plasmid 32a-ORF95.The plasmid was then transformed into E.coli BL21（DE3） strain to induce expression of ORF95 protein.The soluble ORF95 protein can be highly expressed under the induction of IPTG（0.1 mmol·L-1） at 23℃ for 14h,and then purified by Ni-NTA His.Binding affinity purification.The protein can be used further to understand the characteristic of ORF95 protein and the AngHV prevention.