中文摘要：

目的检测乳腺癌组织中水通道蛋白1（AQP1）的表达，分析其表达与乳腺癌临床病理特征和预后的关系。方法应用免疫组织化学sP法检测30例乳腺小叶增生、16例乳腺导管原位癌（Dcts）和78例非特殊型浸润性导管癌（IDC—NOS）组织中AQP1的表达情况，分析AQP1在IDC—NOS细胞质中的表达与患者临床病理特征和预后的关系。结果AQP1在100％的乳腺小叶增生中为肌上皮细胞的细胞膜着色，仅有10．O％的病例伴有腺上皮细胞的细胞质着色。AQP1在100％的DCIS中为肌上皮细胞的细胞膜着色，仅有12．5％的病例伴有腺上皮细胞的细胞质着色。AQP1在35．9％的｜DC-NOS中为肿瘤细胞的细胞质着色，仅有3．8％的病例伴有肿瘤细胞的细胞膜着色。在78例IDC—NOS中，AQP1强阳性表达14例，弱阳性表达14例，阴性表达50例。AQP1在IDC—NOS细胞质中的表达与病理分期、孕激素受体状态、HER-2状态、淋巴结状态、诺丁汉预后指数、有无复发或转移有关（均P〈0．05）。AQP1强阳性、弱阳性和阴性表达IDC．NOS患者的5年无进展生存率分别为16．8％、90．9％和94．9％，差异有统计学意义（P〈0．05）。多因素生存分析的结果显示，淋巴结状态和AQP1的细胞质表达水平是影响IDC·NOS患者无进展生存时间的独立因素（均P〈0．05）。AQP1强阳性、弱阳性和阴性表达IDC—NOS患者的5年总生存率分别为45．6％、90．0％和97．7％，差异有统计学意义（P〈0．05）。多因素生存分析的结果显示，淋巴结状态和AQP1的细胞质表达水平是影响IDC-NOS患者总生存时间的独立因素（均P〈0．05）。结论AQP1在乳腺良性病变组织中主要为肌上皮细胞的细胞膜表达，而在乳腺癌细胞中以细胞质表达为主，其在乳腺癌细胞中的表达是影响患者预后的独立因素。

英文摘要：

Objective To detect the expression of aquaporin 1 （ AQP1 ） in breast cancer tissues, and to analyze its relationship with clinicopathological characteristics and prognosis of breast cancer patients. Methods Histochemical SP staining was used to assess the AQP1 expression in 30 cases of lobular hyperplasia of mammary gland, 16 cases of ductal carcinoma in situ （DCIS） , and 78 cases of invasive ductal carcinoma-not otherwise specified （IDC-NOS）, and to analyze the relationship between cytoplasmic expression of AQP1 in IDC-NOS and clinical pathological characteristics and prognosis of the patients. Results Positive AQP1 immunolabelling appeared as brown deposit over the membrane of myoepithelial cells in all cases of lobular hyperplasia of mammary gland, but only 10.0% of cases showed cytoplasmic staining in glandular epithelial cells. In the ductal carcinoma in situ, brown deposit of AQP1 immunolabelling appeared over the myoepithelial cell membrane in all cases, but only 12.5% of cases were accompanied with cytoplasmic staining in glandular epithelial cells. In the invasive ductal carcinoma not otherwise specified,35.9% of the cases showed cytoplasmic AQP1 tivity, but only 3.8% of cases showed positive membrane staining of the tumor cells. There were highly positive AQP1 expression in 14 cases, weakly positive in ld cases, and negative in 50 cases. Cytoplasmic AQP1 expression in the IDC- NOS cases was significantly correlated with pathologic stage, PR, HER-2, lymph node status, Nottingham prognostic index （NPI） and metastasis or recurrence （all P 〈 0.05）. The 5-year progression-free survival （PFS） rates were 16.8% in the patients with strong positive AQP1 expression, 90.9% in the cases with weakly positive AQP1 expression and 94.9% in the AQPl-negative cases, showing a significant difference （ P 〈 0.05 ）. Multivariate analysis indicated that the lymph node status and cytoplasmic expression of AQP1 were independent factors for PFS （both P 〈0.05）. The 5-year overall survival （