中文摘要：

目的研究2型糖尿病患者血浆中纤维胶凝蛋白3（FCN3）表达趋势。方法利用二维聚丙烯酰胺凝胶电泳分离2型糖尿病和健康对照人群血浆中的蛋白质，并利用MALDI-TOF-TOF鉴定差异蛋白。使用WesternBlot和酶联免疫反应对二维聚丙烯酰胺凝胶电泳的结果进行验证。结果补体C1S亚成分，补体C3、C9以及FCN3在糖尿病患者血浆中浓度升高。WesternBlot结果表明，糖尿病患者血浆中补体c9和FCN3浓度普遍偏高于正常对照人群。ELISA数据显示糖尿病患者血浆FCN3浓度为（50．88±3．85）μg／mL，健康对照人群为（36．20±2．75）μg／mL，差异有统计学意义（P=0．0062）；糖尿病患者血浆中c9浓度为（26．22±1．43）μg／mL，健康对照人群为（19．23±1．55）μg／mL，差异有统计学意义（P=0．0022）。结论2型糖尿病患者血浆FCN3浓度升高，更大程度地激活了补体系统。

英文摘要：

Objective To explore the expression trend of fieolin 3 （FCN3） in type 2 diabetes （T2DM） plasma. Methods Two-dimensional polyacrylamide gel eleetrophoresis （2DE） was used to separate the plasma proteins from T2DM patients and healthy control subjects. MALDI-TOF-TOF was used to identify the differential proteins. Western Blot and enzyme-linked immune response （ELISA） were used to verify the results from 2DE. Results The experiment on 2DE showed complement Cls subcomponent, complement C3, C9 and FCN3 were up-regulated in the plasma of T2DM patients. The Western Blot results showed that C9 and FCN3 increased in the plasma of T2DM patients with respect to T2DM-free subjects. The experiment results on ELISA showed that the plasma concentration of FCN3 were （50. 88 ± 3.85 ） and （ 36.20± 2.75 ） μg/mL （ P = 0. 0062 ） in T2DM and T2DM-free, respectively. The plasma concentration of C9 were （26.22 ＋ 1.43） and （19.23 ＋1.55） μg/mL （P=0.0022） in T2DM and T2DM-free. FCN3 and C9 were up-regulated in T2DM plasma. Conclusion FCN3 over-expressed in the plasma of T2DM patients,which activated the complement system in greater degree.