中文摘要：

目的利用OFFGEL电泳一差异蛋白质组学法，比较肥胖亚型和非肥胖亚型糖尿病患者的差异蛋白，进一步阐释并比较肥胖亚型和非肥胖亚型糖尿病的形成机制。方法分别选择18-44岁男性肥胖亚型、非肥胖亚型2型糖尿病患者及健康对照样本各10例；血浆样品经高特异性免疫亲和法去除白蛋白和IgG后，进行溶液酶切，对多肽混合样品进行OFFGEL电泳；利用NanoHPLC-Chip．MS／MS系统对按等电点分离的多肽样品进行进一步分离和质谱鉴定，比较3组样本中的差异蛋白质，并进行功能注释和机制探讨。结果肥胖亚型、非肥胖亚型糖尿病患者组和对照组分别鉴定391、415、371种蛋白质，组间差异蛋白质比较和蛋白质注释结果表明脂联素在肥胖亚型糖尿病患者血浆中低表达，基质交感分子1在肥胖亚型糖尿病患者血浆中高表达；丝氨酸蛋白激酶ATM、丝氨酸／苏氨酸蛋白激酶WNK1等6种蛋白激酶在非肥胖亚型糖尿病患者血浆中表现出高表达。结论脂联素、基质交感因子1和一系列蛋白激酶在肥胖亚型、非肥胖亚型糖尿病的发病机制上可能存在差异。

英文摘要：

Objective With the increasingly serious epidemic situation of diabetes, plasma proteomic method and OFFGEL electrophoresis have been applied for screening different proteins between obese and non-obese T2DM patients, which may be used to further explain the mechanism of T2DM. Methods Twenty male T2DM volunteers （Obesity Subtype： 10; Non-obesity Subtype： 10） with the age of 18 -44 years have been selected. The control group has been matched considering the factors of age, gender, etc. Albumin and IgG were removed from the plasma samples with highly specific immune- affinity method. Then the peptide-mixed samples were separated by pl with OFFGEL electrophoresis after solution digestion. Further separation and identification were performed by Nano HPLC-Chip-MS/MS system. Comparing the three groups, the differences were obtained and annotated on functions and its mechanism. Results 391, 415 and 371 proteins have been identified in the experimental groups and control group, respectively. The different proteins in groups and their annotations showed that adiponectinwas down-regulated in obesity subtype of T2DM group, while STIM1 （stromal interaction molecule 1 ） was up-regulated. There were six protein kinases high expression in non- obesity DM patients, such as Serine-protein kinase ATM, Serine/threonine-protein kinase WNK1. Conclusion Adiponectin, STIM1 and protein kinases may act in different roles on the pathogenesis of obesity subtype and non-obesity subtype of T2DM