中文摘要：

目的：探讨Ang-1、Ang-2及Tie2mRNA在骨肿瘤组织中的表达及其意义。方法：采用逆转录聚合酶链反应法对正常骨组织、骨软骨瘤、尤文肉瘤和骨肉瘤组织标本各6例内Ang-l、Ang-2及Tie2mRNA含量进行半定量检测。结果：恶性骨肿瘤组织（尤文肉瘤、骨肉瘤）内Ang-2mRNA／Ang-1mRNA比值较正常骨组织和良性肿瘤骨组织比值上调。尤文肉瘤和骨肉瘤中Tie2mRNA的表达上调。结论：恶性骨肿瘤组织内Ang-1，Ang-2及Tie2mRNA表达失衡，肿瘤骨组织内可能存有Ang／Tie2的自分泌和旁分泌系统。深入探讨Ang-1／Ang-2／Tie2在肿瘤骨组织中的分子生物学机制及其临床病理联系，有望使其成为判定骨肿瘤预后的指标，并为骨肿瘤分子治疗提供靶位点。

英文摘要：

Objective： To investigate the expression of Ang-1, Ang-2 and Tie2 mRNA in bone tumor tissues and to explore its clinical significance. Methods： Semi-quantative RT-PCR was used to analyze the expression of Ang-1, Ang-2 and Tie2 mRNA in tissues harvested from normal bone, osteochondroma, Ewing＇s sarcoma and osteosarcoma（n=6）. Results： Ratio of Ang-2 mRNA/Ang-1 mRNA was increased in malignant lesions （Ewing＇s sarcoma and osteosarcoma） when compared with benign lesions （normal bone and osteochondroma）. Expression of Tie2 mRNA was up-regulated in Ewing＇ sarcoma and osteo- sarcoma. Conclusion. The balance of Ang-1, Ang-2 and Tie2 mRNA levels was disturbed in malignant lesions. There may exist a possible autocrine and paracrine pathway of Angs/Tie2 system in bone malignant lesions. Further investigation of Ang1-, Ang-2 and Tie2 and their biomedical significance in bone tumor tissues and clarify their clinical-pathological relation will make them possible prognostic indices and provide molecular therapy target.