中文摘要：

[目的]观察吡咯喹啉醌对雪旺细胞的增殖作用及其对体外培养雪旺细胞NF-κB表达的影响。[方法]新生鼠雪旺细胞体外原代培养及纯化，S-100免疫荧光标记鉴定雪旺细胞，设实验组与对照组，实验组吡咯喹啉醌的浓度为100nmol／L；加药72h后提取总mRNA及总蛋白进行RT—PCR和Western blot分析。[结果]采用RT—PCR和Western blot检测NF—κB的表达，结果均表明实验组表达较对照组高（P〈0．05）。[结论]吡咯喹啉醌可促进雪旺细胞增殖，NF—κB上调在吡咯喹啉醌促雪旺细胞增殖中起作用。

英文摘要：

[ Objective] To investigate the effects of pyrroloquinoline quinine （PQQ） on proliferation and expression of NF - κB of Schwann ceils. [ Methods ] Schwann cells were cultured from sciatic nerves of SD rats in vitro. The Schwann cells were identified and purified by immunofluorescence of S - 100 and Ara - C. Experimental group with was 100 nmol/L of PQQ and control group were set up. The expression of NF - κB in Schwann cells was determined by RT - PCR and Western blotting. [ Resuits] The expression of NF - κB were up - ragulated by PQQ in cultured Schwann cells （P 〈0. 05） . [ Conclusion] PQQ could promote the proliferation of Schwann cells and up - regulation of NF - rd3 play an important role in this process.