中文摘要：

目的：研究阻断NF-kB信号通路对人肝门部胆管癌细胞QBC939生长增殖的影响。方法：常规培养QBC939细胞，采用PDTC（100μmol／L）阻断NF-kB信号通路，Westernblot检测核内P65蛋白水平的表达，CCK-8检测细胞生长增殖抑制率，流式细胞仪观察细胞周期与凋亡的变化情况。结果：经PDTC阻断NF-kB信号通路后，与对照组相比，细胞核内P65蛋白水平明显下降（P〈0．05）。CCK-8检测显示细胞增殖活性明显受到抑制，并随时间的延长而愈渐明显，12、24、36h后细胞增殖抑制率分别为（22．53±0．03）％、（46．54±0．03）％、（58．02±0．03）％，与对照组相比差异有统计学意义（P〈0．05）。流式细胞仪检测发现实验组G0／G1期细胞比例高于对照组，分别为（68．53±1．72）％、（38.03±1．35）％，差异有统计学意义（P〈0．05）；细胞凋亡率在实验组为（27．68±2．77）％，对照组仅为（9．27±2．36）％，差异有统计学意义（P〈O．05）。结论：阻断NF-kB信号通路诱导人肝门部胆管癌细胞GdG，期阻滞及细胞凋亡，从而抑制细胞生长与增殖，提示NF-kB信号通路组成性激活参与人肝门部胆管癌的发生发展，以NF-kB信号通路作为治疗的靶点可能给人肝门部胆管癌带来新的治疗选择：

英文摘要：

Objective： To evaluate the effect of NF-kB pathway interference on the proliferation of QBC939 cells. Methods： Hu- man hilar cholangiocarcinoma QBC939 cells were cultured in RPMI1640 medium, and NF-kB activity was suppressed by PDTC （ 100 μmol/L ）. The P65 subunits of NF-kB in the nuclei of QBC939 cells were detected by Western blot analysis. The inhibition rates of cell growth were assayed by the CCK-8 method. Cell cycles and cell apoptosis were analyzed by flow cytometry （FCM）. Results： The p65 subunits of NF-kB decreased significantly after PDTC （ 100 μmol/L ） treatment （ P 〈 0.05 ）. The CCK-8 assay showed that the prolifer- ation of QBC939 cells was significantly suppressed by PDTC in a time-dependent manner （ P 〈 0.05 ）.The inhibition rates were 22.53%±0.03%, 46.54%±0.03%, and 58.02%±0.03% after 12, 24, and 36 h, respectively. Cell cycle analysis by FCM showed that the percentage of QBC939 cells in the G0/G1 phase was increased by PDTC treatment, i.e., 68.53%±1.72% vs. 38.3%±1.35% （ P 〈 0.05 ）. PDTC induced 27.68% ±2.77% apoptosis compared with 9.27%±2.36% in the controls （ P 〈 0.05 ）. Conclusion： NF-kB func- tions in the cell proliferation and survival of human hilar cholangiocarcinoma QBC939 cells. The inhibition of NF-kB activity induces cell apoptosis and cell cycle arrest in the G0/G1 phase. Thus, targeting the NF-kB signaling pathway can be a new therapeutic strategy for human hilar cholangiocarcinoma.