中文摘要：

背景：这研究的目的是验证简化尖锐生理学分数傻瓜 3 承认分数(傻瓜 3 ) 并且比较它的与在进入一个单个中心的特别护理单位(ICU ) 的病人的一件独立样品的傻瓜 II 的合适。方法：为连续地进入在 2006 年 1 月 1 日和 2007 年 9 月 2 日之间的一所 700 床大学医院的八床的 ICU 的所有成年病人的数据是镇定的。傻瓜 II 和傻瓜 3 被计算，以及预言的医院死亡。傻瓜 II 和傻瓜的刻度 3 根据一般方程(GE ) ，并且为南部的欧洲和地中海国家(SE&MC ) 的方程，和中央、西方的欧洲(C&WE ) ，被 goodness-of-fit Hosmer Lemeshow Ĥ 和 Ĉ 统计估计。有 95% 信心间隔(95% CI ) 的标准化死亡比率(SMR ) 为傻瓜 II 和傻瓜被计算 3 个方程。结果：684 个病人被学习(男性 63%) 。中部的年龄是 73 (quartiles 65 80 ) 年。傻瓜合适 3 使用 C&WE 方程(Ĥ 13.49， P=0.095；Ĉ 12.73， P=0.121 ) 象傻瓜的一样， II 是可接受的(Ĥ 6.02， P=0.644；Ĉ12.08， P=0.147 ) 傻瓜 3 GE (Ĥ 23.36， P=0.002；Ĉ 22.37， P=0.004 ) 并且 S&MC (Ĥ 25.73， P=0.001；Ĉ 26.19， P=0.001 ) 没适合很好。傻瓜 3 GE，傻瓜 3 个 SE&M 国家和傻瓜 II 显著地在上估计了死亡。仅仅为傻瓜的 SMR 的 95% CI 3 C&WE 包括了 1 (SMR 0.97；95% CI 0.89 1.05 ) 。结论：每 ICU 应该识别傻瓜对它的盒子混合很合适的 3 方程。傻瓜 II 模型趋于过高估计死亡。

英文摘要：

AIM： To investigate the role of insulin-like growth factor binding protein-7 （IGFBP-7） in the activation and transdifferentiation of hepatic stellate cells （HSC） in vitro. METHODS： Rat HSC-T6 cells were cultured in separate dishes and treated with various concentration of transforming growth factor （TGF）-β1, IGFBP-7 or anti- IGFBP-7 antibody for 24 h. The supernatant or a cytoplasm suspension was obtained from cultured HSC, followed by transfer of cells to form cell-coated dishes. Immunocytochemistry and Western blotting were used to analyze the expression of IGFBP-7 induced by TGF-β1 and the level of fibronectin, collagen Ⅰ and α-smooth muscle actin （SMA）. The pro-apoptotic effect of anti- IGFBP-7 antibody was determined by flow cytometry. RESULTS： Immunocytochemistry and Western blotting revealed that the expression of IGFBP-7 in TGF-β1 treated HSC was significantly up-regulated compared to that in the control group. In addition, fibronectin, collagen Ⅰ and α-SMA also showed enhanced expression in accordance with the transdifferentiation process in a dose-dependent manner to some extent. Moreover, flow cytometry suggested that anti-IGFBP-7 antibody induced apoptosis of activated HSC, which is responsible for the development of liver fibrosis, and may represent a novel pathway and target for therapeutic intervention. CONCLUSION： IGFBP-7 showed increased expression in activated HSC and played an important role in the activation and transdifferentiation process of HSC. Anti- IGFBP-7 antibody may ameliorate liver fibrogenesis.