中文摘要：

鹅膏肽类毒素是蘑菇中毒导致死亡的最主要因素,用于鹅膏中毒治疗的解毒药物已有大量报道,水飞蓟宾（silibinin,SIL）被认为是目前最有效的解毒药物。灵芝作为我国传统的中药真菌,具有良好的保肝护肝作用。本文开展灵芝主要活性成分灵芝总三萜（Ganoderma total triterpenoids,GTT）和对照药物水飞蓟宾对α-鹅膏毒肽（α-amanitin,α-AMA）中毒小鼠所致肝损伤的保护作用及其抗氧化机理研究,结果表明α-鹅膏毒肽中毒小鼠经灵芝总三萜和水飞蓟宾治疗后小鼠血清中的ALT和AST活性显著降低,小鼠死亡率降低40%–50%,病理组织切片观察表明能明显减轻肝组织损伤的程度,说明灵芝总三萜与水飞蓟宾一样,对α-鹅膏毒肽中毒小鼠的肝具有很好的保护作用。灵芝总三萜给药组和水飞蓟宾给药组能显著增加肝组织中SOD和CAT的活性,显著降低肝脏中MDA含量,表明灵芝总三萜和水飞蓟宾具有抗氧化和自由基清除剂作用,能减轻α-鹅膏毒肽所引起的脂质过氧化伤害作用。

英文摘要：

The cyclopeptide toxins are mainly responsible for the death of humans in the mushroom poisoning. A wide variety of antidotes for amatoxin poisoning have been reported and silibinin（SIL） seemed to be the most effective therapeutic antidotes. Ganoderma lingzhi is a well-known traditional medical species, which exhibited obvious protective effects against liver injury. The aim of this study was to evaluate the hepatoprotective activities of total triterpenoids from G. lingzhi（GTT） on α-amanitin（α-AMA） induced liver injury in mice and analyze the possible mechanisms related to radical scavenging activity. The results showed that treatment with GTT and SIL significantly reduced serum ALT and AST levels and reduced mortality rates 40%–50%. The histopathological observations also showed that treatments with GTT and SIL were able to reduce significantly the liver injury. These data demonstrated that GTT and SIL exhibited hepatoprotective effects on acute liver injury induced by α-AMA. Furthermore, our experimental data show that administration of GTT and SIL could significantly increase SOD and CAT activity and reduce the content of MDA in the liver, indicating that GTT and SIL have potent antioxidative and radical-scavenging effects and could provide effective protection against lipid peroxidation induced by α-AMA.