中文摘要：

樟子松是我国北方的主要造林树种。苗木质量是造林成功与否的关键。木霉菌可以有效促进作物的生长,并能很好地诱导植物产生系统抗性,促进种子萌发和增加根茎生物量。然而,大量研究都是关于农作物,将木霉应用于林木的研究还鲜有报道。为了探明绿木霉菌株T43对樟子松苗木的促生作用及对苗木生理生化指标的影响,采用打孔灌根接种方法对樟子松1年生苗木接种菌株T43,对接种苗木体内过氧化氢酶（CAT）、过氧化物酶（POD）、超氧化物歧化酶（SOD）活性、可溶性蛋白和可溶性糖含量进行动态跟踪测定,在生长季结束时对各处理苗木生物量进行调查。结果表明：与CK相比,经过T43诱导处理后,苗木表现出较高的CAT、POD和SOD活性,其中CAT和POD出现平稳上升的趋势,SOD表现出波动上升的趋势,但这3种酶整体都表现出上升的趋势,且在整个过程中其活性水平均高于CK。与该结果类似,可溶性蛋白和可溶性糖含量也表现出高于CK的现象,不同的是,二者在诱导处理后即出现上升,随着时间的推移,其含量在下降中趋于平稳,但始终高于CK。在生物量方面,T43处理组的苗高、地径、鲜质量和干质量均高于CK,苗木生长质量显著提高。接种3个月后,与对照相比苗高提高了36.7%,地径提高了22.2%,干质量提高了50%,鲜质量提高了21.7%。认为使用绿木霉接种处理苗木促进苗木生长、提高苗木质量是可行的。

英文摘要：

Pinus sylvestris var. mongolica is a major species for forest culture in northern China. The quality of its seedlings is crucial for successful afforestation. Trichoderma spp. can effectively promote the growth of crops, induce systematic resistance of plants and increase seed germination and root and shoot biomass. However, most studies of Trichoderma spp. promotion have focused on crops and only very few on forest tree seedlings. In order to investigate the effects of T. virens on the growth, physiological and biochemical parameters of P. sylvestris var. mongolica seedlings, we incubated 1-year-old seedlings with T. virens T43, by drilling and root irrigation and examined dynamically the activities of catalase （CAT）, peroxidase （POD） , superoxide dismutase （SOD）, as well as the amounts of soluble protein and soluble sugar. At the end of the growing season, we measured the biomass of T43-inoculated T. virens seedlings. Our results indicated that T. virens T43-inoculated seedlings had higher enzyme activity than the non-inoculated control （CK） seedlings throughout the entire trial, with a steady increase for CAT and POD and a fluctuating upward trend for SOD. Similarly, T43 inoculation resulted in higher amounts of soluble protein and soluble sugar than those in the CK throughout the trial, with an immediate increase in enzyme activities after inoculation, followed by a decline to a steady state at a lower level for both treated and non-treated seedlings. The inoculated seedlings also had increases in height （36.7%） , collar diameter （22. 2% ）, dry weight （50%） and fresh weight （21.7%） three months after inoculation compared to the control. Our study suggests that it is feasible to use T. virens T43 to promote the growth and improve the quality of P. sylvestris var. mongolica seedlings.