中文摘要：

目的：探讨精液冷冻保存在少弱精子症患者行宫腔内人工授精（IUI）周期中的应用效果。方法：2008年3月至2009年6月103对原发性不孕夫妇,共计152个IUI治疗周期,其中精液正常者53个周期（组1）,少弱精子者52个周期（组2）,少弱精子经精液冷冻保存后结合新鲜精液者47个周期（组3）。检测治疗前后精液常规、精液处理后前向活动精子总数,随访3组患者治疗期间配偶妊娠结局。结果：精液处理前组3精液体积、精子活率、a级精子均低于组2,差异极显著（P〈0.01）;组3处理后前向活动精子总数高于组2,但差异无显著性（P〉0.05）。3组间生化妊娠率和临床妊娠率均无显著性差异。结论：精液冷冻保存可以增加少弱精子症患者前向活动精子总数,精液冷冻保存技术可以在一定程度上提高其IUI妊娠率。精液冷冻保存与IUI相结合,可能是治疗少弱精子症一种较为理想的方法。

英文摘要：

Objective：To explore the application value of semen cryopreservation in intrauterine insemination cycles for the treatment of oligospermia and asthenospermia in infertile males.Methods：A total of 152 cycles of intrauterine insemination were performed for 103 couples with constitutional infertility,which were divided into Groups 1（normal semen,n = 53）,2（oligospermia and asthenospermia,n = 52）and 3（fresh combined with cryopreserved semen,n= 47）.Semen parameters and forward motile sperm count after processing were recorded by Makler chamber,and all were followed up for the outcome of clinical pregnancy.Results：Compared with Group 2,Group 3 showed obviously lower semen volume,sperm motility and grade a sperm count before semen processing,with extremely significant differences（P0.01）,but a higher rate of grade a sperm after semen processing,though with no significant differences（P0.05）.There were no statistically significant differences in the pregnancy rate between Groups 2 and 3（9.6% versus 14.9%,P 0.05）.Conclusion：Semen cryopreservation can improve the forward motile sperm count and pregnancy rate in oligospermia and asthenospermia patients after intrauterine insemination.Semen cryopreservation combined with intrauterine insemination seems an ideal treatment for oligospermia and asthenospermia in infertile men.