中文摘要：

旨在研究可卡因一苯丙胺调控转录肽（Cocaineamphetamineregulatedtranscript，cART）对促卵泡素（Follicularstimulatinghormone，FsH）诱导的猪卵巢卵泡颗粒细胞雌激素产生的影响。用Long—term培养方法在不同CART、FSH浓度下对猪卵巢卵泡颗粒细胞培养7d，在显微镜下观察各孔细胞生长情况并采集图像；采用双抗体夹心ABC-ELISA法测定各孔雌激素浓度。细胞培养168h后：（1）在培养液不添加CART时，添加FSH孔中的雌激素浓度与不添加孔相比，显著提高；浓度为25ng·mL-1时，雌激素浓度最高，为（17295．57±184．03）Pg·mL^-1；（2）CART对体外培养的无FSH和FSH为5ng·mL-1的猪卵巢卵泡颗粒细胞雌激素的产生没有影响；（3）当培养体系FSH浓度分别为25和50ng·mL^-1时，随着CART浓度的升高（0．01、0．1、1μmol．L^-1），培养液雌激素浓度有下降的趋势，但各组间差异不显著（P〉O．05）。但是，当FSH为25ng·mL^-1，CART为0．1和1μmol．L^-1时，与对照组相比，颗粒细胞雌激素的产生明显受到抑制（P〈0．05）。但是，统计分析FSH和CART互作效应并不显著（P〉O．05），这一点需进一步试验证明；（4）当培养液添加50ng·mL-1FSH时，随着CART浓度的升高（O．01、0．1、1μmol．L^-1），与对照组相比，雌激素浓度各组差异不显著（P〉0．05）。猪卵巢卵泡颗粒细胞雌激素的产生需在FSH诱导下进行；当培养液FSH为25和50ng·mL^-1时，CART对FSH诱导的猪卵巢卵泡颗粒细胞雌激素的产生有抑制作用，但抑制效果并不显著。由此推断，并不像单胎的牛、羊，CART在猪卵泡发育过程中，可能并非是个主要的局部负调控因子。

英文摘要：

The study focused on the effect of CART on FSH-induced estradiol production of por- cine ovarian follicular granulose cells. Granulose cells were cultured for 7 days under different CART and FSH concentrations by Long-term culture. The growth situation in each well was observed and pictures were taken. The content of estradiol in each well was determined by sandwich ABC-ELISA. After 168 h of incubation, （1） In CART-free medium, the estrodiol levels in wells supple- mented with FSH significantly increased than that of FSH-free well. Adding of 25 ng · mL-1 of FSHresulted in the highest estrodiol level of （17 295.57±184.03） pg · mL-1 ; （2） CART showed no effect on estradiol production by porcine ovarian follicular granulose cells cultured in FSH-free and 5 ng· mL-1 FSH medium; （3） In the medium containing 5 or 25 ng ·mL^-1 FSH,with the increase of CART concentration（0.01, 0. 1, 1 μmol.L^-1）, the levels of estradiol concentration showed a tendency to decrease but there was no significant differences among the three groups（P〈 0.05）. However, when FSH at 25 ng · mL-1 , CART at 0.1 or 1 μmol.L^-1 , compared with the control group （FSH 25 ng ~ mL-1 , CART 0 μmol.L^-1） , estradiol secreted by follicular granu- lose cells was suppressed markedly（P〈0.05）. The result of statistical analysis demonstrated no significant interactive effect between FSH and CART （P〈0. 05）, which needs further investiga- tion. （4） When the medium contained 50 ng ·mL^-1 of FSH, the increase of CART concentration showed no significant influence on estradiol concentration in different groups （P〉0.05）. The production of follicular estradiol in pig was induced by FSH ~ when the concentration of FSH were 25 and 50 ng ·mL^-1 , CART inhibited the estradiol production by porcine follicular granulo- sa cells, but no significant inhibitory effects. The result indicate that CART maybe not a main lo- cal negative regulatory factor in pig follicular development.