中文摘要：

目的：探讨生脉注射液和血塞通注射液对大鼠H9c2心肌细胞系缝隙连接蛋白43（Connexin43,Cx43）表达和细胞凋亡的影响。方法：体外培养H9c2细胞，随机分成5组：正常对照组、模型组、血塞通组、生脉组、生脉联合血塞通组。分别以佛波酯（Phorbolesters，e．g．，TPA）作为刺激因子，诱导心肌细胞Cx43损伤；以血管紧张素Ⅱ（AngiotensinⅡ，AngⅡ）作为刺激因子，诱导心肌细胞凋亡。采用免疫细胞化学法检测Cx43的表达，凋亡小体染色检测心肌细胞凋亡率。结果：与正常对照组比较，模型组Cx43表达的面积无显著变化（P〉O．05），平均光密度和积分光密度值显著降低（P〈0．01），凋亡率显著增加（P〈0．01）。与模型组比较，Cx43表达的面积各组间无显著差异（P〉O．05），平均光密度和积分光密度值血塞通组、生脉组和生脉联合血塞通组均显著增加（P〈O．05，P〈O．01），细胞凋亡率血塞通组、生脉组和生脉联合血塞通组均显著降低（P〈0．01）。与血塞通组和生脉组比较，生脉联合血塞通组的细胞凋亡率显著降低（P〈O．05，P〈0．01）。结论：生脉注射液和血塞通注射液可以在一定程度上提高H9c2细胞Cx43的表达，抑制细胞凋亡，二者联合使用时对细胞凋亡的抑制作用更强。

英文摘要：

Objective： To study effects of Shengrnai injection and Xuesaitong injection on expression of connexin 43 and apoptosis in H9c2 cardiac myoblasts. Methods： H9c2 cells in vitro cultured were randomly divided into five groups, including the normal control group, the model group, the Xuesaitong group, the Shengmai group, the combine Shengmai with Xuesaitong group. The maladaptive remodelling of connexin 43 were induced using phorbol esters, e.g., TPA as stimulating factor. And the apoptosis of cardiac myoblasts were induced using Angiotensin II as stimulating factor. The protein expression of connexin 43 was detected by immunocytochemistry. The myocardial apoptosis rate was detected by apoptotic body staining. Results： Compared with the normal control group, the expression area of cormexin 43 had no significant change in model group （P 〉 0.05）, the average optical density and the integral optical density value was decreased significantly in model group （P〈0.01）, and the apoptosis rate was obviously increased （P〈 0.01） in the model group. Compared with the model group, there was no significant difference in the expression area of connexin 43 among different groups （P 〉0.05）. The average optical density and the integral optical density value of connexin 43 expression was increased significantly （P〈0.05, P〈0.01）, and the apoptosis rate was obviously decreased （P〈0.01） in the Xuesaitong group and the Shengmai group and the combined Shengmai with Xuesaitong group. Compared with the Xuesaitong group and the Shengmai group, the apoptosis rate was obviously decreased （P〈0.05, P〈0.01） in the combined Shengmai with Xuesaitong group. Conclusions： Shengmai injection and Xuesaitong injection could improve the expression of Cx43, inhibit apoptosis in H9c2 cells to a certain extent. When combined with two kinds of injection, the inhibition of apoptosis was stronger than that of the single use.