中文摘要：

目的：观察脓毒症大鼠脾脏胆碱能系统酶活性变化与致炎细胞因子TNF-α含量变化的关系,初步探讨脾脏胆碱能系统对炎症反应的调控作用.方法：采用盲肠结扎穿孔（CLP）方法制备大鼠脓毒症模型.动物随机分为对照组及CLP6、12和24 h 4组.于术后相应时间点取脾脏,测定脾脏TNF-α含量及胆碱乙酰基转移酶（chAT）、乙酰胆碱酯酶（AchE）的活性.结果：大鼠脾脏chAT和AchE活性在CLP后逐渐增加,于CLP12 h达峰值,CLP24 h 时有所回落;chAT/AchE活性比值在CLP后呈增加趋势;TNF-α含量与chAT活性变化呈正相关（r=0.94,P〈0.05）.结论：脓毒症发展过程中,大鼠脾脏非神经性胆碱能系统参与调控炎症反应.

英文摘要：

Objective： To observe the relationship between the activities of cholinergic related enzymes and tumor necrosis factor-α （TNF-α） content in spleen of rats with sepsis, and to explore the modulating effect of splenic non-neuronal cholinergic on inflammation. Methods：Sepsis model was reproduced in rats by cecum ligation and puncture （CLP）. The rats were randomly divided into sham operation （control）, CLP6 hours, CLP12 hours, and CLP24 hours groups. The splenic choline acytyltransferase （chAT） and acetylcholinesterase （AchE） activities were determined with spectrophotometry, and TNF-α content was determined by enzyme-linked immunoadsorbent assay （ELISA）. Results： After CLP, both chAT and Ache activities in the spleen were gradually increased, the activities of the enzymes reached to the peak level at 12 hours after CLP, and lowered to certain extent at CLP 24 hours. The relative activity of chAT/AchE showed an elevation tendency. Change in TNF-α content in spleen was in accordance with that of chAT activity（r= 0.94, P〈0. 05）. Conel~ions： During the development of sepsis, the splenic non-neuronal cholinergic system is involved in regulating the inflammatory reaction.