以簸箕柳×绵毛柳的F1代为作图群体,构建1份柳树遗传连锁框架图,该图谱包含117个标记(56个SSR,54个SRAP,5个SCAR和2个性别标记),图谱总图距为1631.4cM,标记间平均图距为14.1cM。估算柳树基因组的大小为2261.39cM,总的图谱覆盖率为72.14%。2个性别标记Sex-F和Sex-M被定位于该图谱第1和第17连锁群上,在第1连锁群上存在与雌性性别共分离的标记位点SCAR_AE08-780-5。通过基因组图谱初步比较研究发现,柳树框架图与毛果杨全基因组之间共有46个同源标记、15个同源连锁群,80.95%的同源标记具有共线性,这说明杨柳科基因组在进化过程中具有较高的保守性。
Based on a mapping population derived from Salix suchowensis × S.erioclada F1 family,a genetic frame map of willow with 117 markers(56 SSR,54 SRAP,5 SCAR and 2 sex markers) was constructed in this study.The map was totally 1 631.4 cM with average distance between markers of 14.1cM and covered 72.14% of the total length of the genome which was estimated to be as long as 2 261.39 cM.Two sex markers were located on group 1 and 17 and the marker SCAR_AE08-780-5 was co-segregated with the female marker Sex-F on group 1.Alignment of this frame map to the Populus trichocarpa physical map revealed 46 homologous markers,15 homological linkage groups and 34 of 42 homologous markers(80.95%) were at high co-linearity.Overall,the genome of the Salicaceae family was well conserved in the course of evolution.