中文摘要：

为探讨雏鸡口服不同浓度黄芪多糖（APS）后肠道分泌型IgA^＋抗体生成细胞（IgA^＋细胞）的分布情况,将80只1日龄雏鸡随机分为4组：3组试验组和1组对照组,每组20只,10日龄起对3个试验组雏鸡分别定时口服免疫1、2、4 mg/m L的APS 0.5 m L,对照组雏鸡以相同方式口服相同剂量的生理盐水,连续免疫4 d。于末次免疫后第1、7、14、21、28天,从每组中分别随机选取4只雏鸡,取其相同部位的十二指肠肠段,采用免疫组织化学双重染色方法分析十二指肠内IgA^＋细胞的变化情况。结果显示,试验组雏鸡十二指肠IgA^＋细胞表达率明显高于对照组,具有显著差异或极显著差异（P〈0.05或P〈0.01）;不同剂量黄芪多糖免疫雏鸡后三者间的结果差异显著（P〈0.05）。本试验结果表明,口服黄芪多糖可增强雏鸡十二指肠黏膜的免疫功能。

英文摘要：

The study aims at the changes of chicken duodenum IgA^＋positive cells after oral APS. The chicks were randomly divided into four groups： three immune groups and one control group. Each group has 20 chicks.Ten-day-old chickens in immune groups were orally immunized with 0.5 m L APS at a dosage of 1 mg/m L, 2 mg/m L and 4 mg/m L once a day for 4 days,respectively. The control group were administered saline and treated as the same way as immune groups. Four chicken duodenums were randomly removed from each group after the last day of immunization on day 1,7,14,21 and 28. Using immunohistochemical method double staining the changes of IgA^＋positive cells were analyzed in chicken duodenum. The results indicated that APS could increase the number of IgA^＋positive cells in which immune groups were significantly higher than the control group（P〈0.05 or P〈0.01）; different doses of APS had significant difference（P〈0.05）. It is concluded that oral administration of APS could enhance duodenum mucosal immune function of chicken.