中文摘要：

为了观察不同日龄鸡鼻黏膜及其淋巴组织（NALT）的形态结构,从而了解其不同时期的免疫状态。选择不同日龄的鸡胚和雏鸡,每个时期取3只鸡胚或雏鸡的鼻组织制成冰冻切片,片厚5μm,然后进行H.E.染色。结果发现,胚胎18日龄即可辨认鼻腺结构;出壳后1日龄时,鼻内黏膜形态结构发生变化,表面形成假复层柱状纤毛上皮;7日龄时,鼻泪管口处首先出现淋巴集合体（NALT）;21日龄时,鼻后孔处呼吸黏膜中也形成了发达的NALT,且初次发现淋巴小结,此外在鼻甲黏膜中也形成明显的NALT;35日龄时,鼻黏膜厚度显著增加,几乎达到成熟水平,鼻腺管周围也出现NALT,而且在鼻腺管上皮下存在大量弥散性淋巴细胞。表明鸡鼻黏膜以及NALT的组织结构随着日龄增长逐渐发育成熟,并在35日龄时基本达到成熟水平。

英文摘要：

The aim of this study was to investigate the histological structure of nasal mucosa and nasal-associated lymphoid tis-sue（NALT）in chickens at different ages.In this study,different ages of chicken embryos and chicks were chosen, nasal tissue of threeeggs or chicks in every stage were collected and frozen.The frozen tissues were sectioned for 5 μm thick,and then subjected to HEstaining.The result showed that nasal gland could be observed clearly at 18 old embryo.Mucosal morphology of nasal changeed at 1day post-hatch,that is pseudostratified columnar ciliated epithelium emerged;Lymphatic aggregates were firstly occurred at Nasolac-rimal duct mouth at 7 day post-hatch,that means nasal associated lymphoid tissue（NALT）has formed; When it was 21 days post-hatch,NALT has also emerged at the choana of chicken,meanwhile lymphoid nodule could be observed for the first time;NALTemerged around nasohypophysial duct,additionally at 35 days post-hatch,many Diffuse lymphoid cells located behind epithelium ofnasohypophysial duct.Histological structure of nasal mucosa and NALT in chickens developed to maturity gradually with the increaseof age,and the immune function reached the mature level basically at 35-day-old.