中文摘要：

开发一只兔子的目的更确切模仿人的 intervertebral 圆盘退化的病理学的变化的 intervertebral 圆盘退化当模特儿。12 只新西兰白兔子被利用建立损害根据下列协议建模的三个不同磁盘的方法；组织 A：anulus 刺在 L2-L3 和 L5-L6 用完了一根 18 计量器针；组织 B：有在 L3-L4 的一根 23 计量器针的 interleukin-1 IL-1 尾的 intradiscal 注射；并且组织 C：磷酸盐缓冲区的 intradiscal 注射盐(PBS ) 与在 L4-L5 的一根 23 计量器针。L1-L2 水平被用作控制。兔子在 24 个星期以后被打死。intervertebral 圆盘高度被侧面的平凡的拍测量。在 X 光线照相术的大小被获得以后， intervertebral 磁盘为 DNA， sulfated glycosaminoglycan (s 作呕) 和原子核 pulposus 的水内容被移开并且分析。intervertebral 圆盘高度， s 作呕，和水在 anulus 针刺满足的结果显著地在组 A，而是 DNA 被减少在原子核 pulposus 的内容显著地被增加什么时候与控制相比。磁盘高度和水内容的重要减少被表明，仅仅 s 作呕和 DNA 内容没在组 B 看重要差别是否与控制相比。圆盘高度， s 作呕，水，和 DNA 内容的重要减少没在组 C 显示出是否与控制相比。18 计量器刺建模的结论在兔子生产了最一致的圆盘退化腰部的脊骨。

英文摘要：

Objectives： To develop a rabbit model of intervertebral disc degeneration that more exactly simulates the pathological changes of human intervertebral disc degeneration. Methods： Twelve New Zealand white rabbits were utilized to establish three different disc injury models according to the following protocol; group A： anulus punctures were done with a 18-gauge needle at L2-L3 and L5-L6; Group B： intradiscal injection of interleukin-1 IL-1β with a 23-gauge needle at L3-L4; and Group C： intradiscal injection of phosphate buffer saline（PBS） with a 23-gauge needle at L4-LS. The L1-L2 level was used as a control. Rabbits were killed after 24 weeks. The intervertebral disc height was measured by lateral plain radiographs. After the radiographic measurements were obtained, the intervertebral discs were removed and analyzed for DNA, sulfated glycosaminoglycan（s-GAG） and water contents of nucleus pulposus. Results： The intervertebral disc height, s-GAG, and water contents in anulus needle punctures were significantly decreased in Group A, but the DNA content in the nucleus pulposus was significantly increased when compared to the control. The significant decrease of disc height and water contents were demonstrated, only the s-GAG and DNA contents did not show a significant difference in Group B when compared to the control. The significant decrease of disc height, s-GAG, water, and DNA contents did not show in Group C when compared to the control. Conclusion： The 18-gauge puncture models produced the most consistent disc degeneration in the rabbit lumbar spine.