中文摘要：

客观：为了探索骨头的幸存和迁居，间充质的干细胞移植 ated 在内部兔子的脊椎的圆盘和 exogenic genes.Methods 的表示：32 只兔子被使用，一个使随机化的块图案被使用，在一样的兔子的磁盘是一块，到 L5-6 的从 L2-3 的腰部的磁盘随机被划分成空白的组，盐的组，房间移植组Ⅰ和房间移植组Ⅱ。荧光显微镜学被用来决定制造者蛋白质 GFP 的荧光， DNA-PCR 被用来在 1 点分析新霉素抵抗的基因的 DNA 的拷贝， 3， 6，在 transplantation.Results 以后的月：在我和在空白的 II 和没有组织的房间移植组有荧光，在 1 点的盐的组， 3，在移植以后的 6 个月。在房间移植组，荧光灯的分发是更多与时间，而是房间组Ⅰ和Ⅱ之间的没有重要差别散布。抵抗基因在房间移植组Ⅰ和Ⅱ和定量分析表示了的新霉素的测试证明房间组Ⅰ和Ⅱ之间没有重要差别(P 】 0.05 ) 。结论：移植骨头间充质的干细胞能幸存，移居，转移基因能高效地表示，它建议 BMSC 治疗可能是有效的阻止并且对待内部脊椎的圆盘退化。

英文摘要：

Objective： To explore the survival and migration of bone mesenchymal stem cells transplantated in intervertebral disc of rabbits and expression of the exogenic genes. Methods. Thirty-two rabbits were used, A randomized block design was used and discs in the same rabbit were one block,the lumbar discs from L2-3 to L5-6 were randomly divided into blank group, saline group, cell transplantation group Ⅰand cell transplantation group Ⅱ. The fluorescence microscopy was used to determine the fluorescence of the maker protein GFP and DNA-PCR was used to analyze the copies of DNA of neomycin-resistant gene at 1, 3, 6, months after transplantation. Results： There was fluorescence in cell transplantation group Ⅰ and Ⅱ and none in blank group, saline group at 1, 3, 6 months after transplantation. In cell transplantation groups,the fluorescent distribution was more scatter with time, but no significant difference between cell groups Ⅰ and Ⅱ. The test of neomycin resistant gene expressed in cell transplantation group Ⅰ and Ⅱ and quantitative analysis showed that there was no significant difference between the cell groups Ⅰ and Ⅱ （P〉0.05）. Conclusion： The transplanted bone mesenchymal stem cells can survive, migrate and the transfer genes can express efficiently, it suggests that the BMSC therapy may be effective to prevent and treat intervertebral disc degeneration.