中文摘要：

有 remifentanil 的背景 Preconditioning 授与 cardioprotection。因为 Ca2+ 超载是损害的一个猛抛的因素，我们在细胞内部的 Ca2+ 上决定了 remefentanil 的效果([Ca2+] 我) 并且它在老鼠的电的刺激和咖啡因导致的 transients，反映由处理蛋白质的 Ca2+ 处理的 Ca2+ ，室的 myocytes。刚孤立的成年男 Sprague-Dawley 老鼠 myocytes 与 Fura-2/AM 被装载的方法并且[Ca ] 我被 spectrofluorometry 决定。在 0.1-1000 g/L 的 Remifentanil 被管理。在管理以后的十分钟，也， 0.2 Hz 电的刺激被使用或 10 mmol/L 咖啡因被增加。[Ca2+]我，和振幅，休息的时间和两 transients 的50%腐烂( t50 )由电的刺激导致了( E [Ca2+]我)并且咖啡因( C [Ca2+]我) determined.Results Remifentanil ( 0.1-1000.0 g/L )被减少[Ca2+]我以一种剂量依赖者方式。它也减少了两 transients 的振幅 dose-dependently。而且，达到顶点增加了时间，两 transients dose-dependently.Conclusion Remifentanil 的 t50 减少了[Ca2+] 我并且压制了电的刺激和咖啡因在老鼠导致的 transients 室的 myocytes。

英文摘要：

Background Preconditioning with remifentanil confers cardioprotection. Since Ca^2＋ overload is a precipitating factor of injury, we determined the effects of remefentanil on intracellular Ca^2＋ （[Ca^2＋]i） and its transients induced by electrical stimulation and caffeine, which reflects Ca^2＋ handling by Ca^2＋ handling proteins, in rat ventricular myocytes. Methods Freshly isolated adult male Sprague-Dawley rat myocytes were loaded with Fura-2/AM and [Ca]i was determined by spectrofluorometry. Remifentanil at 0.1-1000 μg/L was administered. Ten minutes after administration, either 0.2 Hz electrical stimulation was applied or 10 mmol/L caffeine was added. The [Ca^2＋]i, and the amplitude, time resting and 50% decay （t50） of both transients induced by electrical stimulation （E[Ca^2＋]i） and caffeine （C[Ca^2＋]i） were determined. Results Remifentanil （0.1-1000.0 μg/L） decreased the [Ca^2＋]i in a dose-dependent manner. It also decreased the amplitude of both transients dose-dependently. Furthermore, it increased the time to peak and tso of both transients dose-dependently. Conclusion Remifentanil reduced the [Ca^2＋]i and suppressed the transients induced by electrical stimulation and caffeine in rat ventricular myocytes.