中文摘要：

目的观察母源性BDE-209暴露后仔鼠海马组织结构形态及海马Ca^2＋／钙调蛋白依赖性蛋白激酶Ⅱ（CaMKⅡ）含量的变化。方法3月龄Wistar大鼠自妊娠0d起按照随机数字表法分为实验组和对照组（E组），实验组母鼠在妊娠期及哺乳期给予不同剂量BDE-209（100、300、600、1200mg／kg）胃灌，分别为A组、B组、C组、D组；E组胃灌等量花生油，21d仔鼠断乳后各组选取15只雄鼠，HE染色后光镜观察仔鼠海马组织的变化，ELISA法检测海马CaMKⅡ含量。结果HE染色后光镜下观察发现C、D组仔鼠海马组织形态学变化明显，神经元数量明显减少。随着暴露剂量增加，仔鼠海马CaMKⅡ含量逐渐下降，C、D组与E组比较差异有统计学意义（P〈0．051，A、B组与E组比较差异无统计学意义（P〉0．05）。实验组间比较，A组与D组差异有统计学意义（P〈0．05），B、C组与D组差异无统计学意义（P〉0．05）。结论高剂量母源性BDE-209暴露可以导致仔鼠海马组织结构发生改变，神经元数量减少，CaMKⅡ含量下降，影响仔鼠神经系统的发育。

英文摘要：

Objective To study the effect of maternal deca-brominated diphenyl ether （PBDE-209） exposure on the microstructure and calmodulin-dependent protein kinase Ⅱ （CaMK Ⅱ ） content in the hippocampus of the offspring rats. Methods Three-month-old female Wistar rats were exposed to peanut oil suspensions of commercial BDE-209 administered by daily oral gavage at the doses of 100, 300, 600 and 1200 mg/kg （groups A, B, C, and D, respectively）. The control rats （group E） were given only peanut oil of the same volume. The microstmcture of the hippocampus of 5 offspring rats from each group was observed microscopically using HE staining. Ten offspring rats from each group were examined for CaMK Ⅱcontent in hippocampus using enzyme-linked immunosorbent assay. Results Obvious histomorphological changes were found in the hippocampus of the offspring rats in groups C and D. The CaMK Ⅱ content in the hippoeampus decreased with the increase of the doses of BDE-209 exposure, and compared with group E, the offspring rats in groups C and D showed significantly decreased CaMK Ⅱ content in the hipposcampns （P〈0.05, respectively）, but those in groups A and B showed no significant variations in CaMK Ⅱ content （P〉0.05, respectively）. Among the experimental groups, compared with group D, the offspring rats in group A showed significant decreased in CaMK Ⅱ （P〈0.05）, but those in group B and C showed no significant variations （P〉0.05）. Conclusion Maternal BDE-209 exposure can induce histological changes in the hippocampus, decrease the neuronal number, and lower CaMK Ⅱ content in the hippocampus of the offspring rats to affect the development of the nervous system.