中文摘要：

为建立血水草的RAPD-PCR最佳反应体系,对影响血水草RAPD反应的Mg2＋、模板DNA、dNTPs、引物浓度和Taq聚合酶用量等因素进行优化。结果表明：25μL反应体系中含10×Buffer 2.5μL,1.8 mmol.L-1Mg2＋,2UTaqDNA聚合酶,50 ng模板,0.2 mmol.L-1dNTPs,1.6μmol.L-1引物。扩增程序为：94℃预变性2 m in;预扩增：94℃变性20 s,36℃退火30 s,72℃延伸75 s,5个循环;扩增：94℃变性20 s,40℃退火30 s,72℃延伸60 s,40个循环;72℃保温20 m in,4℃保存。所建立的血水草RAPD-PCR反应体系具有标记位点清晰、反应系统稳定、检测多态性能力强、重复性好等特点,可以较好地应用于血水草的遗传多样性分析研究。

英文摘要：

In order to establish the optimal reaction of RAPD-PCR amplification for Eomecon chionantha Hance,the following parameters were optimized： the concentrations of Mg2＋,template DNA,dNTPs,primers and Taq DNA polymerase.The optimal RAPD-PCR reaction system was as follows a total volume of 25 μL contains 2.5 μL 10×Buffer,1.8 mmol·L-1 Mg2＋,2 U Taq DNA polymerase,50 ng template DNA,0.2 mmol·L-1 dNTPs and 1.6 μmol·L-1 primer.The reaction program fitting to the RAPD-PCR was as follows： initial denaturation at 94℃ for 2 min,followed by 5 beforehand cycles of 94℃ for 20 s,36℃ for 30 s,72℃ for 75 s;followed by 40 cycles of 94℃ for 20 s,40℃ for 30 s,72℃ for 60 s,and a final exposure to 72℃ for 20 min,then stored in 4℃.This RAPD-PCR system has some distinguising features including clear marker site,stable reaction system,reliable abundant polymorphisms,and better repeatability.It is suitable for the study on genetic diversity of E.chionantha.