中文摘要：

目的：通过建立饥饿诱导的自噬模型,探讨CD147对前列腺癌PC-3细胞自噬作用的影响,并阐明其作用机制。方法：通过饥饿诱导方式建立自噬细胞模型。实验分为阴性对照组和RNAi干扰CD147组（PC-3/shCD147组）。GFP-LC3质粒转染观察细胞自噬斑点形成情况,免疫印迹技术检测自噬蛋白LC3和Beclin-1表达,台盼蓝排斥实验检测细胞死亡率。结果：与阴性对照组比较,PC-3/shCD147组GFP-LC3斑点细胞数增加（P〈0.01）,自噬相关蛋白LC3-Ⅱ（P〈0.01）和Beclin-1（P〈0.05）表达水平升高;与阴性对照组（22.3%±3.5%）比较,PC-3/shCD147组细胞死亡率（38.4%±3.1%）明显升高（P〈0.05）。结论：在前列腺癌PC-3细胞中,CD147通过Beclin-1抑制饥饿诱导的自噬过程,减少细胞自噬的发生。

英文摘要：

Objective： To establish starvation-induced autophagy model and explore the influence of CD147 in autophagy of prostate cancer PC-3 cells, and to clarify its mechanism. Methods.. Starvation method was employed to establish autophagy model. The experiment included negative control group and CD147 shRNA group （PC-3/ shCD147）. The autophagy spot formation was detected by GFP-LC-3 plasmid transfection, and the LC3-11 and Beclin-1 protein expressions were determined by Western blotting method. Tryan blue exclusion assay was used to analyze the cell death. Results： Compared with negative control group, the number of GFP-LC3 spot cells in PC-3/ shCD147 group was increased （P〈0. 01）, and the expression levels of LC3-11 and Beclin-1 were also increased （P〈0.05 or P〈0.01）. Meanwhile, the cell death rate in PC-3/shCD147 group （38. 4%±3.1%） was increased compared with negative control group （22.3%±3.5%） （P〈0. 05）. Oonclusion.. CD147 can inhibit the starvationinduced autophagy with Beclin-1, and decrease the autophagy death in prostate cancer PC-3 cells.