中文摘要：

目的探讨白细胞分化抗原（CD147）对前列腺癌细胞雄激素受体（AR）磷酸化的影响及其作用机制。方法选择前列腺癌LNCaP细胞,随机分为LNCaP/Scramble组、LNCaP/sh CD147组,分别感染阴性对照病毒、CD147RNA干扰病毒,采用Western blotting法检测沉默CD147表达效率。收集两组细胞,加或不加磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（AKT）信号通路抑制剂LY294002处理,采用Western blotting法检测磷酸化AKT（p-AKT）、磷酸化AR（p-AR）和前列腺特异性抗原（PSA）表达。结果 LNCaP/Scramble组CD147相对表达量为1.19±0.09,LNCaP/shCD147组为0.71±0.03,两组比较P〈0.01。与LNCaP/Scramble组比较,LNCaP/shCD147组p-AKT、p-AR和PSA相对表达量均明显降低（P均〈0.05）;加入PI3K/AKT信号通路抑制剂LY294002处理后,LNCaP/sh CD147组p-AKT、p-AR和PSA相对表达量进一步下降（P均〈0.05）。结论沉默CD147可降低AR磷酸化水平,其机制可能与影响PI3K/AKT信号通路有关。

英文摘要：

Objective To investigate the effects of leukocyte differentiation antigen（ CD147） on phosphorylation of androgen receptor（ AR） in prostate cancer cells and its mechanism. Methods The prostate cancer LNCaP cells were selected and then were randomly divided into the LNCaP/Scramble group and LNCaP/sh CD147 group. LNCaP cells in the above two groups were transfected with the negative control virus and CD147 interference virus,respectively. The CD147 expression was detected by Western blotting. The expression of phosphorylated protein kinase B（ p-AKT）,phosphorylated androgen receptors（ p-AR） and prostate specific antigen（ PSA） in cells of the two group treated with or without LY294002 was detected by Western blotting. Results The expression of CD147 in the LNCaP/Scramble group was 1. 19 ± 0. 09,versus 0. 71 ± 0. 03 in the LNCaP/sh CD147 group（ P〈0. 01）. We found that the expression of p-AKT,p-AR,and PSA was decreased in the LNCaP/sh CD147 group as compared with that of the LNCaP/Scramble group（ all P〈0. 05）. When cells were treated with LY294002 for 24 h,the expression of p-AKT,p-AR,and PSA protein was significantly decreased in the LNCaP/Scramble group（ all P〈0. 05）. Conclusion CD147 can cause phosphorylation of AR through PI3K/AKT pathway and then increase PSA expression in prostate cancer,which facilitates prostate cancer progress.