为了探究白头翁水溶性多糖的提取工艺,本研究采用单因素试验和L9(34)正交试验,考察料液比、提取时间、提取次数和提取温度对水溶性多糖提取率的影响,优选白头翁水溶性多糖的最佳提取工艺条件。结果表明,白头翁水溶性多糖最佳提取工艺条件为:料液比为1:17,100℃提取6 h,提取6次,白头翁水溶性多糖含量为5.07%。为白头翁水溶性多糖的进一步研究奠定了基础。
To optimize the extraction procedure of water-soluble polysaccharides from Radix Pul-satilla, single factor test and orthogonal test were designed to investigate the effects of sol-id-liquid ratio, extraction time and number of extraction. The results showed that the optimal ex-tracting conditions were the solid-liquid ratio of 1:17, extraction time of 6 hrs and extracting 6 times at 100 ℃ in water. Under these conditions, the extraction percentage was up to 5.07%. This study provided the basis for further research of water-soluble polysaccharides in Radix Pulsatilla.