中文摘要：

为了探讨纳米SiO2和常规SiO2颗粒对Hela细胞的细胞毒性作用，采用不同浓度的纳米SiO2和常规SiO2颗粒（0．05、0．1、0．2、0．4、0．8、1．6μg·μL^-1）对Hela细胞进行12h染毒，应用MTT法检测细胞毒性效应．研究发现，较低浓度（≤0．2μg·μL^-1）的纳米SiO2和常规SiO2对Hela细胞无明显细胞毒性（P〉0．05）；较高浓度时，纳米SiO2（≥0．4μg·μL^-1）和常规SiO2（≥0．8μg·μL^-1）对Hela细胞具有明显细胞毒性作用（p〈0．01），并且随浓度增大细胞毒性增强；当浓度≥0．4μg·μL^-1时，纳米SiO2的细胞毒性明显高于相同浓度的常规SiO2（p〈0．05）．以上结果表明，纳米SiO2和常规SiO2颗粒均能对Hela细胞产生细胞毒性，且纳米SiO2的细胞毒性强于常规SiO2；低浓度（≤0．2μg·μL^-1）的纳米SiO2和常规SiO2具有很好的生物相容性．

英文摘要：

In order to explore and compare the cytotoxicity induced by nano-SiO2 and normal SiO2 particles, Hela cells were exposed to these two particles with different concentrations （0.05、0.1、0.2、0.4、0.8、1.6μg·μL^-1） for 12h. The MTT assay was applied to measure the cytotoxicity. Results showed that, low concentration （≤〈0.2μg·μL^-1） nano-SiO2 and normal SiO2 had no significant toxicity to Hela cells（p〉0.05）. While in the high concentration, nano-SiO2 （≥0.4μg·μL^-1） and normal SiO2 （≥0.8μg·μL^-1）had significant toxicity to Hela cells （p〈0.01）, and the cytotoxicity increased with the increase of concentrations. Nano-SiO2 induced greater cytotoxicity than normal SiO2 at the same concentration（p〈 0.05 ） when the concentration was above 0.4μg·μL^-1. It was concluded that both nano-SiO2 and normal SiO2 particles could induce cytotoxicity to Hela cells, and nano-SiO2 showed greater cytotoxicity than normal SiO2 particles. Low concentration（ ≤ 0.2μg·μL^-1） nano-SiO2 and normal SiO2 were well biocompatible with Hela cells, and this characteristic probably had a good application foreground in biomedical research.