中文摘要：

为了探讨纳米水滑石与Hela细胞的生物相容性，采用单细胞凝胶电泳法检测了纳米水滑石对Hela细胞DNA的损伤，并采用H2DCF—DA荧光法检测了纳米水滑石对Hela细胞活性氧簇的影响。结果发现，随着纳米水滑石浓度的升高（0—800μg．mL^-1），Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量均呈逐渐升高趋势；较低浓度（50、100μg．mL^-1）纳米水滑石处理后，Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量与对照组无显著性差异（P〉0．05），而较高浓度（200、400、800μg．mL^-1）纳米水滑石处理后，Hela细胞尾部DNA含量、尾距及胞内活性氧簇含量与对照组差异显著（p〈0．05，p〈0．01），以上结果表明，较高浓度（≥200μg．mL^-1）的纳米水滑石可对Hela细胞DNA产生损伤，而较低浓度（≤100μg．mL^-1）的纳米水滑石则具有较好的生物相容性，有可能作为载体应用于医药领域。

英文摘要：

In order to explore the biocompatibility of nano-layered double hydroxides （nano-LDHs） with Hela cells, a single cell gel electrophoresis and H2DCF-DA fluorescent coloration assay were used to analyze the DNA damage and the change of reactive oxygen species （ROS） in Hela cells exposed to nano-LDHs. Results showed that, with the increase of concentrations of nano-LDHs （0-800μg.mL^-1）, the Tail DNA%, Tail Moment and ROS contents in Hela cells increased gradually; There were significant differences （p〈0.05, p〈0.01） in Tail DNA%, Tail Moment and ROS contents between the high concentration groups （200, 400, 800μg.mL^-1） and the control group, while no significant differences （p〉0.05） were found between the low concentration groups （50, 100μg.mL^-1） and the control group. The above results suggested that the high concentration nano-LDHs （≥200μg.mL^-1） could induce the DNA damage in Hela cells and the low concentration nano-LDHs （≤100μg.mL^-1） had a good biocompatibility with Hela cells.