中文摘要：

调查 decabromodiphenyl 乙烷(DBDPE ) 的有毒的效果的目的，在 vitro 用作 decabromodiphenyl 醚的一种选择。方法 HepG2 房间分别地为 24， 48，和 72 h 面对在各种各样的集中(3.125100.0 mg/L ) 的 DBDPE 是有教养的， DBDPE 的有毒的效果被学习。由 3-(4,5-dimethylthiazol-2-yl ) 评估了 -2,5-diphenyl tetrazolium 溴化物的结果并且喂奶脱氢酶试金和原子词法变化， DBDPE 在 12.5 mg/L 的一个范围以内在一个时间依赖者和剂量依赖者举止禁止了 HepG2 生存能力到 100 mg/L 并且为 48 h 和 72 h。apoptosis 的正式就职被 propidium 碘化物染色在 48 h 和 72 h 在 12.5100 mg/L 检测，与反应的氧种类(ROS ) 的生产过剩伴随了。而且， N-acetyl-L-cysteine，广泛地使用的 ROS scavenger，显著地减少的导致 DBDPE 的 ROS 层次和增加的 HepG2 房间生存能力。结论 DBDPE 有细胞毒素并且反增长效果和罐头导致 ROS 在起一个重要作用的 apoptosis

英文摘要：

Abstract Objective To investigate the toxic effects of decabromodiphenyl ethane （DBDPE）, used as an alternative to decabromodiphenyl ether in vitro. Methods HepG2 cells were cultured in the presence of DBDPE at various concentrations （3.125-100.0 mg/L） for 24, 48, and 72 h respectively and the toxic effect of DBDPE was studied. Results As evaluated by the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase assays and nuclear morphological changes, DBDPE inhibited HepG2 viability in a time- and dose-dependent manner within a range of 12.5 mg/L to 100 mg/L and for 48 h and 72 h. Induction of apoptosis was detected at 12.5-100 mg/L at 48 h and 72 h by propidium iodide staining, accompanied with overproduction of reactive oxygen species （ROS）. Furthermore, N-acetyI-L-cysteine, a widely used ROS scavenger, significantly reduced DBDPE-induced ROS levels and increased HepG2 cells viability. Conclusion DBDPE has cytotoxic and anti-proliferation effect and can induce apoptosis in which ROS plays an important role