中文摘要：

目的：观察HH胶囊治疗HBV转基因小鼠抗乙肝病毒（HBV）疗效及其作用机制。方法：HBV转基因小鼠随机分为4组,苦参素组小鼠给予同体积含150mg·kg^-1·d^-1苦参素混悬液,高、低剂量HH胶囊组小鼠分别给予同体积含7、2g·kg^-1·d^-1HH混悬液,5周后,ELISA法检测血清及肝组织乙型肝炎病毒表面抗原（HBs Ag）,常规HE染色观察肝组织病理变化,定量PCR法检测血清中乙型肝炎病毒脱氧核糖核酸（HBVDNA）及肝组织中HBVDNA、TANK结合激酶1（TKB1）m RNA、干扰素调控因子7（IRF7）m RNA表达。结果：高剂量HH胶囊可以改善肝细胞轻度脂肪变性、肝细胞轻度水肿及Kuffer细胞增生等病理改变,对肝组织炎细胞浸润未见明显缓解。与模型组比较,高剂量HH胶囊（7g·kg^-1·d^-1）可以显著降低HBV转基因小鼠肝脏和血清中的HBs Ag、HBVDNA水平,同时显著增强小鼠TKB1 m RNA、IRF7 m RNA表达,差异具有统计学意义（P〈0.01,P〈0.05）。结论：HH胶囊具有抗HBV作用,推测可能与增强TKB1 m RNA、IRF7 m RNA表达进而影响干扰素分泌有关。

英文摘要：

Objective： To observe the curative effect of HH Capsule on hepatitis B virus（HBV） of HBV transgenic mice, and explore its action mechanism. Methods： HBV transgenic mice were randomly divided into 4 groups, mice in kushenin group were treated with kushenin mixed suspension（150mg · kg^-1·d^-1）, and mice in high and low dosage of HH Capsule groups were treated with HH mixed suspension（7g · kg^-1·d^-1 and 2g · kg^-1·d^-1）. After treating for 5 weeks, the levels of hepatitis B virus surface antigen（HBs Ag） in serum and hepatic tissue were tested by using ELISA method, and hepatic pathology was observed by using HE staining method. The m RNA expression of hepatitis B virus DNA（HBVDNA） in serum and hepar, TANK-binding kinase1（TKB1） and interferon regulatory factor 7（IRF7） in hepar was tested by using q PCR. Results： High dosage of HH Capsule could improve mild fatty degeneration, mild edema, and proliferation of Kuffer cells in hepatic tissue, and its effect on inflammatory cell infiltration in hepatic tissue was not significant. High dosage of HH Capsule could reduce the levels of HBs Ag and HBVDNA in hepatic tissue and serum（P〈0.01, P〈0.05）, and increase the expression of TKB1 m RNA and IRF7 m RNA of HBV transgenic mice significantly（P〈0.01, P〈0.05）. Conclusion： The effect on anti-HBV of HH Capsule might relate with its interference on internal secretion which was caused by the invigorating effect on the expression of TKB1 m RNA and IRF7 m RNA.