中文摘要：

为探究鸡干扰素基因刺激蛋白（STING）的结构及功能,采用RT-PCR方法从三黄鸡外周血淋巴细胞中扩增出全长STING基因编码区序列并进行克隆测序。序列分析显示,该基因编码区全长1 140bp,编码379个氨基酸。核苷酸序列比较发现,三黄鸡STING基因与原鸡和火鸡的同源性较高,分别为100%和92.3%,与其他物种STING基因的亲缘关系依次为其他禽类〉哺乳动物〉鱼类。预测STING的分子质量为42.63ku,理论等电点为6.67,有较好的亲水性与抗原性。蛋白质二级结构中折叠占7.4%,无规则卷曲占51.2%,螺旋占41.4%。该蛋白由胞内区、跨膜区和胞外区组成,跨膜结构域仅有1个且位于N端,无信号肽。本研究首次成功克隆了三黄鸡STING基因,并对其进行了生物信息学分析,可以为全面解析鸡STING的分子结构及功能提供参考。

英文摘要：

To know the structure and function of STING gene in chicken,the coding region of STING gene was amplified from peripheral blood mononuclear cells of Sanhuang chicken by RT-PCR and cloned, then sequenced.Sequence analysis showed that the coding region of STING gene was 1 140 bp in length and encoded a protein of 379 amino acids.Similarity analysis of nucleotide sequence showed that Sanhuang chicken shared high nucleotide similarity with Gallus gallus and turkey in STING gene （100% and 92.3% ,respectively）.The descending nucleotide similarity orders among Sanhuang chicken and other species were other avian types〉mammals〉fish.The predicted molecular weight and isoelectric point of STING were about 42.63 ku and 6.67,respectively.And the predicted STING protein had high hydrophilicity and antigenieity. The protein structure study indicated that beta-turn, random coil and alpha-helix were 7.4% ,51.2% and 41.4%,respectively.The protein was composed of intracellular domain, transmembrane domain and extracellular domain and just had a transmembrane domain inits N terminal. The protein contained no signal peptide.The STING gene of Sanhuang chicken was cloned successfully for the first time in our study and the bioinformatics analysis was conducted, which provided references for the analysis of molecular structure and function of STING in chickens.