中文摘要：

采用RT-PCR扩增鸡传染性支气管炎病毒（IBV）广西优势血清型代表性毒株Gx-YL5的膜（M）蛋白基因，将其克隆至pFastBacTM／HBM-TOPO杆状病毒转移载体，转化大肠杆菌DHl0Bac感受态细胞，获得重组杆粒rHBM-M；将重组杆粒转染Sf9昆虫细胞，获得重组杆状病毒；利用间接免疫荧光试验（IFA）和蛋白质免疫印迹（Western-blot）鉴定表达的M蛋白。IFA检测可见重组杆状病毒感染后72h细胞出现特异性荧光，Western blot检测可见大小约为26ku的特异性蛋白带，表明该重组M蛋白在Sf9昆虫细胞中成功获得表达。研究为IBVM蛋白的生物学功能、IBV诊断试剂和新型疫苗制备等奠定基础。

英文摘要：

Membrane （M）protein gene of Guangxi representative dominant serotype isolate of avian infectious bronchitis virus （IBV）was amplified by RT-PCR and cloned into pFastBacTM/HBM-TOPO to obtain recombinant transposon vector. The recombinant transposon vector was transformed into DH10Bac to gain recombinant bacmid rHBM-M. The recombinant bacmid rHBM-M was transfected into Sf9 insect cells to obtain recombinant baculovirus. The recombination M protein was detected by indirect immunofluorescence assay （IFA）and Western blot. The results showed that the specific fluorescence was observed in Sf9 insect cells at 72 h post infection of the recombinant baculovirns by IFA and a specific protein band of 26 ku was observed by Western blot, which was indicated that the recombinant M protein was successfully expressed in Sf9 insect cells. It＇s laid a good foundation for study the biological function of IBV M protein, the development of IBV diagnostic reagents and new vaccine.