从微型浮游生物和小型浮游生物中选取两个代表藻种中肋骨条藻(SkeletonemaCOstotum)和海洋原甲藻(Prorocen—trumnzicarl)进行培养和测量,并获得生物一光学数据,分析了两者后向散射光谱变化特性及其影响因素。分析结果表明,以420ifIn处为例,中肋骨条藻的后向散射系数变化范围为0.009~0.097;而海洋原甲藻的变化范围为0.011~0.017,得出小颗粒中肋骨条藻各波段的后向散射变化范围相对较大,但是其单位叶绿素浓度的后向散射系数值除620nm处,其余波段均较海洋原甲藻低;此外,两种藻的后向散射系数与叶绿素浓度均呈良好的线性关系,但是,中肋骨条藻的后向散射光谱形状会随叶绿素浓度的变化而发生改变,而海洋原甲藻的光谱形状则始终保持不变;同时两者的后向散射比率光谱也存在差异,中肋骨条藻的峰值出现在442nm处,而海洋原甲藻的峰值则出现在488nm处。
Variability of the backscattering characteristics about the alga Skeletonema costatum and prorocentrum micans which represent for the nanoplankton and microplankton respectively were examined. The results indicate that the particulate backscattering coefficient value of Skeletonema costautm range between 0. 009 and 0. 097 at 420 nm, while the value of P. micans range between 0.011 and 0. 017, it concludes that the backscattering coefficient variation range of the S. costatum is relatively larger, while it's bbpc value which is the backscattering coefficient that would be exhibited by cells at a concentration corresponding to 1 mg Chllorophytl a, is lower than P. micans at all bands except 620 nm. Otherwise, the backscattering coefficient is highly line correlated with the chlorophyll concentration of the two alga, the shape of the particulate backscattering coefficient spectra of S. costatum is changed from the low chlorophyll concentration to the higher concentration, but the shape of P. micans remain the same. At the same time, there is some difference in the backscattering ratio spectra of the two alga, the maximum of S. costatum appears at 442 nm,while the P. micans'appears at 448 nm.