中文摘要：

通过层层自组装的方法,在中性条件下利用静电作用将Fe3O4纳米粒子组装到修饰了高分子聚电解质的CaCO3多孔微米球上。该复合材料具有好的生物相容性、导电性、磁性和稳定性。将血红蛋白酶固定到该复合材料上,进而制备得到血红蛋白（Hb）-Fe3O4-CaO3复合物修饰玻碳电极,并在该修饰电极上实现了Hb与电极之间的直接电化学。该生物传感器对H2O2的还原具有较好的响应,线性范围为3.0×10-6∽5.3×10-5 mol/L,检测限为8.9×10-7 mol/L。

英文摘要：

A Fe3O4-CaCO3@polyelectrolyte hybrid material was synthesized by a stepwise layer-by-layer method in a neutral aqueous solution through electrostatic interaction.This hybrid material possessed better biocompatibility,electroconductivity,magnetism and stability compared with original CaCO3.The material was further used to immobilize hemoglobin（Hb）.The resulted Hb-Fe3O4-CaCO3@polyelectrolyte composite was used for the decoration of carbon glass electrode（GCE）.Electrochemical tests showed that the direct electrochemistry between protein and the electrode was realized.The biosensor fabricated exhibited a fast amperometric response to hydrogen peroxide（H2O2）with a linear range of 3.0×10-6-5.3×10-5 mol/L.The detection limit was 8.9×10-7 mol/L.