中文摘要：

将室温离子液体1-丁基-3-甲基咪唑四氟硼酸盐（[BMIM]BF4）、N,N-二甲基甲酰胺（DMF）、及不同种类的膜材料与各种酶一起通过混合涂布、逐层修饰的方式固定于三维有序大孔金膜电极表面,构建一种新型的生物酶传感器。血红蛋白（Hb）、肌红蛋白（Mb）和辣根过氧化物酶（HRP）在该修饰电极上呈现出明显的还原峰,这归因于三种酶活性中心的直接电化学行为。分别构建四种不同膜材料的修饰电极,研究了硅凝胶、壳聚糖、Nafion膜和琼脂糖水凝胶四种材料对于L-乳酸脱氢酶（LDH）修饰电极催化响应的影响,结果表明选择壳聚糖作为膜材料是最优选择。将壳聚糖修饰的LDH金膜电极用做检测乳酸浓度的生物传感器,该传感器表现出良好的催化性能,线性响应范围为10∽250nmol/L,检测限（S/N=3）为3.3nmol/L。

英文摘要：

By casting method,room temperature ionic liquids（1-butyl-3-methylimidazolium tetrafluoroborate,N,N-dimethylformamide）,different kinds of film materials and redox proteins have been mixed and further used to fabricate three-dimensional ordered macroporous gold film modified electrodes.The electrodes modified with hemoglobin,myoglobin and Horseradish peroxides showed well-defined reduction peaks in cyclic voltammograms,which could be attributed to the direct electron transfer between the enzyme and the electrode.The effect of different film materials,such as silicone gel,chitosan（Chi）,Nafion and agarose hydrogel,on the electrocatalytic behaviors of lactate dehydrogenase（LDH）modified electrode was also investigated.The experimental results indicated that Chi was the optimum material.Chi modified LDH electrode was used as an electrochemical sensor for the detection of lactate,which showed a wide dynamic range from 10-250nmol/L with a detection limit of 3.3nmol/L（S/N=3）.