中文摘要：

目的探讨氟对大鼠成骨细胞胰岛素样生长因子-1（IGF-1）和受体mRNA、蛋白表达的影响。方法应用酶消化法分离SD大鼠成骨细胞，取第2代成骨细胞，加入不同浓度[0（对照）、10-7、10-6、10-5、10-4、10-3mol／L]的氟（FL），在培养24、48h时采用免疫放射法检测成骨细胞培养液中IGF-1含量：荧光定量PCR法检测成骨细胞IGF-1受体mRNA表达；Westernblot法检测成骨细胞IGF-1受体蛋白表达。结果①随染氟剂量增加，培养24、48h的成骨细胞培养液中IGF-1含量有先升后降的趋势，24h时10μmol／L组IGF-1含量[（65．45±4．84）ng／L]最高，与对照组[（38．83±3．48）ng／L]比较，差异有统计学意义（P〈0．05）；48h时10-5mol／L组IGF-1含量[（59．14±1．53）ng／L]最高，与对照组[（33．79±1．84）ng／L]比较，差异有统计学意义（P〈0．05）。②24、48h10-5mol／L组成骨细胞IGF-1受体mRNA表达（0．0055±0．0004、0．0262．±0：0040）明显高于相应对照组（0．0022士0．0001、0．0073±0．0008，P均〈0．05）。③随染氟剂量增加，培养24、48h的成骨细胞IGF-1受体蛋白表达有先升后降的趋势，其中24h10-5 mol／L组（1．39±0．16）与对照组（0．86±0．12）比较，差异有统计学意义（P〈0．05），48h各染氟组与对照组比较，差异无统计学意义（P均〉0．05）。结论氟可影响大鼠成骨细胞IGF—l和受体mRNA、蛋白表达，提示IGFs信号传导通路系统对氟调控骨代谢有重要作用。

英文摘要：

Objective To explore the influence of fluorine on mRNA and protein expression of the insulin-like growth factor-1 （IGF-1 ） and its receptor of rat osteoblasts. Methods Osteoblasts were isolated from rat bone by enzyme digestion. Different fluorine concentration [ 0 （control）, 10.7, 10-6, 10-5, 10-4, 10-3 mol/L ] was add to the second generation osteoblasts. The IGF-1 in the culture medium was determined by radioimmunoassay （RIA） at different fluorine concentration and different time（24, 48 h）. The expression of IGF-1 receptor was measured by the method of fluorescent quantitation PCR and the expression of protein IGF-1 receptor was measured by Western blotting. Results ①With increased dose of fluoride exposure, IGF-1 concentration in the osteoblastic culture medium increased first and then decreased at 24, 48 h, respectively. Compared to the control group [（38.83 ±3.48）ng/L], IGF-1 concentration of the 24 h 10-6 mol/L group[ （65.45 ± 4.84）ng/L] was higher, and the difference was statistically significant（P 〈 0.05）. The same result was also shown in the 48 h 10-5 mol/L group [ （59.14 ± 1.53 ） ng/L ] to its corresponding control group [ （33.79 ±1.84） ng/L, P 〈 0.05 ]. ②The mRNA expression of IGF-1 reeptor of the 24, 48 h 10-5 mol/L groups （0.0055 ±0.0004, 0.0262 ± 0.0040） was significantly higher than their corresponding control groups （0.0022 ± 0.0001, 0.0073 ± 0.0008, all P 〈 0.05）. ③ With increased dose of fluoride exposure, the protein expression of IGF-1 receptor increased first and then decreased; the expression of 24 h 10-5 mol/L group （1.39 ± 0.16） was compared with the corresponding control group （0.86 ± 0.12）, and the difference was statistically significant （P 〈 0.05）; the expression of 48 h every fluorine group was also compared with the corresponding control group, and the difference was not statistically significant（all P〉 0.05）. Conclusions Fluorine can affect the mRNA and protein expression of osteoblastie IGF-1 an