目的 测定地椒中总黄酮的含量并研究其清除DPPH自由基的活性。方法 采用紫外可见分光光度法测定地椒提取物中总黄酮的含量;用DPPH自由基清除法测定地椒中总黄酮的抗氧化活性。结果 不同产地、采收期地椒药材中总黄酮的含量差异较大,结合抗氧化实验结果可见:甘肃庆阳产地椒中总黄酮的含量最高,最佳采收期为9月。结论 所用方法简便、准确,可作为地椒中总黄酮的含量测定方法;产地和采收期对地椒中总黄酮的含量影响较大;地椒总黄酮具明显的体外抗氧化活性。
OBJECTIVE To establish a method for the determination of total flavones in Thymus quinquecostatus Celak and to study the free radicals scavenging activity. METHODS The content of total flavones in T. quinquecostatus was determined by UV - vis spectrophotometry and the antioxidant capability of total flavones was measured by DPPH assay. RESULTS The content of total flavones has significant difference in the different habitats and harvest time. Comparing with the DPPH free radical - scavenging activity,the content of total flavones was highest in Qingyang of Gansu province and the best harvest time was September. CONCLU- SION The method is simple and accurate,and it can be used as a content determination method for total flavones in T. quinquecosta- tus. The content of total flavones has difference in the different habitats and harvest times and it had obvious antioxidant capability.