中文摘要：

梭梭是生长在我国西北荒漠地区抗逆性极强的一种生态保护型植物。通过RT-PCR技术克隆到梭梭的转录因子HaDREB2C的cDNA全长编码区为1 125 bp（登录号为KU523927）,编码374个氨基酸,其中含有一个AP2结构域;半定量RT-PCR表达量显示,该基因受干旱,高盐的诱导;将HaDREB2C克隆到酵母表达载体pGBKT7中转入AH109酵母细胞,转化子可以在SD-Trp、SD-Trp-His-Ade多营养缺陷型固体培养基中正常生长,运用β-半乳糖苷酶的滤纸分析法显色测定,证明pGBKT7下游报告基因被激活,HaDREB2C在酵母中具有转录激活活性。

英文摘要：

Haloxylo nammodendron is a highly stress-resistance and conservation-type plant that grown in the northwest desert of China. By RT-PCR technique, a ammodendron transcription factor HaDREB2C （KU523927） was cloned. It encodes 374 amino acids, contains a AP2 domain and the full length of coding region is 1 125 bp. Semi-quantitative RT-PCR expression showed that the gene was induced by drought and high salt. After cloning DREB2C into the yeast expression vector pGBKT7 and transferring the vector into AH109 yeast cells, the transformants can grow in SD-Trp and SD-Trp-His-Ade auxotrophy solid medium normally. By using filter paper analysis method of 13-galactosidase chromogenic assay, it was proved that the downstream reporter gene of pGBKT7 vector was activated and HaDREB2C had transcriptional activation.