中文摘要：

以梭梭无菌苗带腋芽茎段作为外植体，诱导出丛生芽，获得梭梭再生苗，并对培养条件进行系统优化。结果表明：腋芽诱导培养基为MS ＋ 蔗糖 30 g/L＋2,4 D 2 mg/L＋6 BA 1 mg/L＋Gelzan 2 g/L＋MgCl21.97 g/L＋PVP 0.4 g/L；分化与增殖培养基为MS＋蔗糖10 g/L＋NAA 3 mg/L＋AgNO3 3 mg/L＋IBA 2 mg/L＋Gelzan 2 g/L＋MgCl21.97 g/L＋PVP 0.4 g/L；壮苗培养基为MS＋蔗糖20 g/L＋2,4 D 1.0 mg/L＋6 BA 1.0 mg/L＋GA3 1.0 mg/L＋Gelzan 2 g/L＋MgCl21.97 g/L＋PVP 0.4 g/L；生根培养基为1/4 MS＋蔗糖 10 g/L＋IBA 1.5 mg/L＋NAA 0.15 mg/L＋Gelzan 2 g/L＋MgCl21.97 g/L＋PVP 0.4 g/L＋活性炭 0.5 g/L。

英文摘要：

Aseptic stems with axillary buds from of Haloxylon ammodendron were used as explants, multiple shoots were induced, and regenerated plantlets were obtained. The plant in vitro culture regeneration system of Haloxylon ammodendron had been optimized. As a result the culture media were as the following. Primary culture medium：MS＋sucrose 30 g/L＋2,4 D 2 mg/L＋6 BA 1 mg/L＋Gelzan 2 g/L＋MgCl21.97 g/L＋PVP 0.4 g/L；differentiation inducing medium of axillary buds： MS＋sucrose 10 g/L＋NAA 3 mg/L＋AgNO3 3 mg/L＋IBA 2 mg/L＋Gelzan 2 g/L＋MgCl21.97 g/L＋PVP 0.4 g/L；sound seedling medium： MS＋sucrose 20 g/L＋2,4 D 1.0 mg/L＋6 BA 1.0 mg/L＋GA3 1.0 mg/L＋Gelzan 2 g/L＋MgCl21.97 g/L＋PVP 0.4 g/L；rooting medium：1/4MS＋sucrose 10 g/L＋IBA 1.5 mg/L＋NAA 0.15 mg/L＋Gelzan 2 g/L＋MgCl21.97 g/L＋PVP 0.4 g/L＋activated carbon 0.5 g/L.