欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
真核生物CDT2是CUL4-DDBlE3泛素复合体的组成部分，在细胞周期调控、DNA复制与损伤修复中起到重要作用．本研究克隆了番茄CDT2同源基因片段并构建了CD丁2基因RNA干涉植物表达载体pBll21-CDT2-RNAi．通过根癌农杆菌介导转入番茄子叶，经组织培养成功获得转基因植株．半定量RT-PCR分析显示，转基因植株叶片内CDT2的表达量明显低于野生型植株．转基因植株叶片叶绿素含量比野生型明显升高．该研究结果揭示番茄CDT2基因的功能做出了新的尝试．
英文摘要：
The eukaryote CDT2 gene is part of CUL4-DDB1 E3 ubiquitin complex. They play an important role in cell cycle, DNA replication and repair regulation. In this study, homologue CDT2 gene was cloned in tomato and a RNA interference （RNAi） vector of CDT2 was constructed. The construct was introduced into tomato cv. Ailsa Craig by means of Agrobacterium-mediated transformation. Analysis of semi-quantitative RT-PCR revealed a distinct reduction of endogenous CDT2 expression levels in the RNAi transgenic lines compared to that of wild-type plants. The Chlorophyll content of leaves in the RNAi repression lines was significantly elevated. The results show us a new way to modify fruit tional quality in tomato through gene engineering.
同期刊论文项目
同项目期刊论文
SlNAC1, a stress-related transcription factor, is fine-tuned on both transcriptional and post-transl
Plant programmed cell death caused by an autoactive form of Prf is suppressed by co-expression of th
A conserved unusual posttranscriptional processing mediated by short, direct repeated (SDR) sequence
Uniform ripening (U) encodes a Golden 2-like transcription factor regulating tomato fruit chloroplas
Rice choline monooxygenase (OsCMO) protein functions in enhancing glycine betaine biosynthesis in tr
Functional characterization of a tomato COBRA-like gene functioning in fruit development and ripenin
The tomato UV-damaged DNA-binding protein-1 (DDB1) is implicated in pathogenesis-related (PR) gene e
期刊信息
位置：