中文摘要：

采用基于纳米纤维固相萃取的高效液相色谱-荧光测定（HPLC-Flu）法测定头发中的皮质醇水平。以新型纳米纤维萃取柱（PFSPE）提取头发样本浸出液,用100μL乙醇洗脱,再加入230μL浓H2SO4反应2 min,反应物加水混合后用PFSPE柱提取,最后用50μL甲醇直接洗脱后注入色谱体系进行分析。此方法的检出限为0.05 ng/g（S/N=3）,日内精密度和日间精密度分别低于6.8%和8.9%,平均方法回收率为88.9%。本方法提取过程简便、快速、环保,而且可以准确测定头发样本中的皮质醇水平。

英文摘要：

In recent years,many studies found that hair cortisol can serve as a good biomarker of chronic stress.A high performance liquid chromatography-fluorescence（HPLC-Flu） method for the determination of hair cortisol based on novel packed nanofiber solid phase extraction（PFSPE） was established.The incubation solution of hair samples was extracted by the new PFSPE column.The column was eluted with 100 μL ethanol,and then 230 μL concentrated sulfuric acid was add in the eluant and retained for 2 min.The mixture of reactants and water was secondly extracted by the PFSPE column,which was eluted with 50 μL methanol directly.The detection limit of cortisol was 0.5 ng/g（signal-to-noise ratio of 3）.The within-run and between-day precisions（RSD） were below 6.8% and 8.9%,and the average recovery of cortisol spiked into hair samples was 88.9%.The method of pretreatment is simple,fast and green.Moreover,the established method can accurately determine the level of cortisol in hair samples,which provides a simple,inexpensive determination method for physiology,psychiatry,sports medicine,cognitive science,psychology and other researches.