中文摘要：

目的应用实时荧光定量PCR技术研究艾滋病患者及正常人肠道拟杆菌属的变化．揭示艾滋病对肠道微生态的影响及其在发病中的作用。方法：收集艾滋病患者（艾滋病组）及正常人（正常对照组）各30例的粪便。根据细菌的靶基因序列设计特异性引物，提取粪便细菌DNA．应用实时荧光定量PCR测定拟杆菌属中6种细菌的数量。结果：正常对照组与艾滋病组粪便中脆弱拟杆菌为[3．23±1．59；4．05±1．65（单位：logN／0．5 g湿便，下同）]，单形拟杆菌为（5．69±0．95；6．70±2．18），多形拟杆菌为（5．01±1．61；6．41±2．34），卵形拟杆菌为（5．78±1．03；7．07 ±1．75），吉氏拟杆菌为（4．21±1．21；5．53 ±2．46），普通拟杆菌为（2．92±1．30；4．48±1．32）．艾滋病组较正常对照组数量均明显增多，差异有统计学意义（p〈0．05）。结论：艾滋病患者肠道拟杆菌较正常对照组增多，提示了艾滋病患者肠道微生态紊乱，为下一步研究及临床诊断治疗提供了理论依据。

英文摘要：

Objective To investigate the bacteroides of patients with AIDS and the healthy control by real-time quantitative PCR in order to reveal the role and significance of gut microflora in the AIDS-associated molecularpathogenesis. Methods The feces of the preoperative AIDS patients （n = 30） and the healthy control （n = 30）were collected. According to the sequences of target genes, specific PCR primers were designed. Bacterial genomeDNA extracted from fecal samples was quantified by real-time quantitative PCR to analyze the bacterial amounts.Results In the patients with AIDS, the level of B.fragilis （3.23 ± 1.59; 4.05 ± 1.65）, B.uniforrnis （5.69 ± 0.95;6.70 ± 2.18）, B.thetaiotaomicron （5.01± 1.61; 6.41 ± 2.34）, B.ovatus （5.78 ± 1.03; 7.07± 1.75）, B.distasonis（4.21± 1.21 ; 5.53 ±2.46） and B.vulgatus （2.92 ± 1.30; 4.48 ± 1.32） were significantly higher than those of healthycontrols （P 〈 0.05）. Conclusion The amount of fecal bacteroides of AIDS patients are significantly higher thanthose of the healthy control. These data indicate that the gut microflora of AIDS patients was disordered.