中文摘要：

目的研究中药单体丹酚酸B对骨髓间充质干细胞（MSCs）中干细胞因子（stemcell factor,SCF）的影响。方法用贴壁培养法分离、培养和纯化MSCs,取第三代MSCs进行实验。用含不同浓度丹酚酸B的低糖DMEM培养液培养MSCs,ELISA法检测培养24、48、72h的上清中SCF的含量;RT-PCR法检测培养24、48、72h的SCF mRNA的表达。结果膜型和可溶型SCF mRNA均有表达。随着培养时间的增加,0.3μg/ml、3μg/ml、30μg/ml丹酚酸B可明显促进SCF的分泌和SCF mRNA的表达（与空白对照组比较,P〈0.05）,而0.03μg/ml丹酚酸B组和空白对照组比较无明显差异（P〉0.05）。结论丹酚酸B可促进MSCs分泌SCF并促进SCF mRNA的表达,提示SCF可能在中药丹参治疗心肌梗死中发挥了重要作用。

英文摘要：

Objective To observe the effects of salvianolic acid B on the stem cells factor （SCF） secretion and mRNA expression of mesenchymal stem cells （MSCs） in vitr. Methods MSCs were isolated, cultured and purified in vitro by adherent culture The 3rd passage of MSCs was chosen to undergo the experiments. MSCs were cultured in five groups of lower glucose DMEM media containing different concentration of salvianolic acid B as follows： group of control （no salvianolic acid B added）, four groups of salvianolic acid B treatment （0. 03μg/ml, 0. 3μg/ml, 3μg/ml and 30μg/ml of salvianolic acid B were added, respectively, into the lower glucose DMEM media）. The quantities of SCF were measured by ELISA in the supernatant of cultured MSCs harvested at 24h, 48h and 72h culturing. The expression of SCF mRNA in the supernatant of cultured MSCs harvested at 24h, 48h and 72h culturing were analyzed with reverse transcription-polymerase chain reaction. Resalts RT-PCR assay displayed that dissolvent and membrane type of SCF mRNA expressed in MSCs basically. The expression of SCF mRNA and the quantities of SCF increased significantly （compared with the control group, P〈（0. 05） in the supernatant of cultured MSCs treated with salvianolic acid B at concentration of 0.3μg/ml, 3μg/ml and 30μg/ml groups. No significant difference existed between the treatment group of salvianolic acid B at 0. 03μg/ml concentration and the blank control group （P〉0. 05）. Condlusion Salvianolic acid B may promote the secretion of SCF and reinforce the expression of SCF mRNA of MSCs, implying that SCF might play an important role on the treatment of myocardial ischemia with the traditional Chinese medicine Salviae miltiorrhizae.