中文摘要：

调查 electroacupuncture (EA ) 是否能支持房间幸存并且提高间充质的干细胞(MSC ) 的心功能的目的治疗。方法 MSC 从骨头髓被孤立并且在最小的必要中等 Alpha 膨胀了(-MEM) 。MI 被左前面的下降冠的动脉(男孩) 的结扎为 30 min 和灌注在 72 只 Sprague-Dawley (S-D ) 老鼠导致。MI 老鼠随机收到了 1 的注射

英文摘要：

Objective： To investigate whether electroacupuncture （EA） can promote cell survival and enhance heart function of mesenchymal stem cells （MSCs） therapy. Methods： MSCs were isolated from bone marrow and expanded in Minimum Essential Medium Alpha （α-MEM）. MI was induced in 72 Sprague-Dawley （S-D） rats by ligation of the left anterior descending coronary artery （LAD） for 30 min and reperfusion. MI rats randomly received injection of 1 x 106 Dil-labeled MSCs alone （n=24, MSC group）, or plus electroacupuncture （EA） at Neiguan （PC6, n=24, EA＋MSC group）, or saline （n=24, saline group）. EA treatment was performed for 4 days. Another 24 rats were subjected to chest-open surgery without LAD occlusion and treatment （sham group）. Three time points, 4, 14 and 28 days （n=8 for each group） were included in this study. The survival of transplanted MSCs and the protective gene expression were analyzed by reverse transcriptase polymerase chain reaction （RT-PCR） and Western blot at day 4 and 14. Left ventricular remodeling, cardiac function, infarction area, fibrosis and capillary density were analyzed at day 28. Results： EA can enhance MSC survival （2.6-fold up） at day 4. Big capillary density was 53% higher in EA＋MSC treated group than MSC alone group. Furthermore, the rats treated by EA reduced the fibrosis and had 36% smaller infarct size comparing to MSC alone. EA also attenuated left ventricular remodeling and enhanced the functional recovery of infarcted hearts at week 4. Conclmion： EA at Neiguan acupoint can promote the stem cell survival and improve ischemic heart function. EA could become a useful approach in stem cell therapy for ischemia heart diseases.