目的:探讨当归多糖、何首乌多糖、熟地多糖、枸杞多糖、黄芪多糖及其含药血清对骨髓间充质干细胞(MSCs)增殖的影响。方法:分离、培养大鼠MSCs,各中药多糖及其含药血清,分别加入培养基诱导72h后,MTT法检测大鼠MSCs增殖情况。结果:1mg/mL黄芪多糖及其10%含药血清对大鼠MSCs增殖的影响,与相应浓度的空白组及空白血清组比较,差异有显著性意义(P〈0.05)。结论:黄芪多糖及其含药血清可促进大鼠MSCs增殖。
Objective:To discuss the effect of angelica polysaccharide,fleece-flower root polysaccharide,rehmannia polysaccharide,medlar polysaccharide,astragalus polysaccharide and their medicated serum on bone marrow-derived mesenchymal stem cells ( MSCs) proliferation. Methods:MSCs were isolated from rat bone and cultured under different polysaccharide and their medicated serum for 72h. After the induction,the proliferation effect on MSCs was detected with MTT. Results:Compared with the control group and blank serum group,the proliferation effect of 1mg/mL astragalus polysaccharide and 10% astragalus polysaccharide medicated serum on rat MSCs,the difference was significant. Conclusion:Astragalus polysaccharide and its medicated serum can promote the proliferation rate of MSCs.