采用纸层析法与紫外分光光度法,对来源于禾本科植物的64株内生固氮菌产γ-氨基丁酸的特性进行分析鉴定.筛选到3株γ-氨基丁酸产量较高的内生固氮菌:Y28(0.754mg·mL^-1)、Y21(0.597mg·mL^-1)、W3(0.539mg·mL^-1).菌株Y28的产量比国内同行研究者初筛选到的产γ-氨基丁酸菌的产量(0.55mg·mL^-1)都要高,兼具固氮活性,具有很高的研究及应用价值.
The gama-aminobutyric acid production ability of sixty-four endophytic diazotrophs from Gramineae were tested by paper chromatography and ultraviolet spectrophotometer. Strains Y28, Y21 and W3 can produce the gama-aminobutyric acid (0. 754 ±0. 004), (0. 597 ±0. 001 ) and (0. 539 ±0. 002) mg·mL^-1, respectively. Diazotrophic strain Y28 can produce the highest gama-aminobutyric acid than that of known description and may be potential application.